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71. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie (DGNC)
9. Joint Meeting mit der Japanischen Gesellschaft für Neurochirurgie

Deutsche Gesellschaft für Neurochirurgie (DGNC) e. V.

21.06. - 24.06.2020

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Immunmodulatory effects of irradiation in the genesis of meningioma

Immunmodulatorische Effekte von Bestrahlung in der Meningeomentstehung

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  • presenting/speaker Marise Walter-Diessars - Universitätsklinikum Jena, Klinik für Neurochirurgie, Jena, Deutschland
  • Diana Freitag - Universitätsklinikum Jena, Klinik für Neurochirurgie, Jena, Deutschland
  • Werner Dammermann - Medizinische Hochschule Brandenburg - Theodor Fontane, Innere Medizin II, Brandenburg an der Havel, Deutschland
  • Lukas Hain - Universitätsklinikum Jena, Klinik für Neurochirurgie, Jena, Deutschland
  • Nasrin Abbasi-Senger - Universitätsklinikum Jena, Klinik für Strahlentherapie und Radioonkologie, Jena, Deutschland
  • Tilo Wiezorek - Universitätsklinikum Jena, Klinik für Strahlentherapie und Radioonkologie, Jena, Deutschland
  • Andrea Wittig - Universitätsklinikum Jena, Klinik für Strahlentherapie und Radioonkologie, Jena, Deutschland
  • Rolf Kalff - Universitätsklinikum Jena, Klinik für Neurochirurgie, Jena, Deutschland
  • Jan Walter - Klinikum Saarbrücken, Klinik für Neurochirurgie, Saarbrücken, Deutschland; Universitätsklinikum Jena, Klinik für Neurochirurgie, Jena, Deutschland

Deutsche Gesellschaft für Neurochirurgie. 71. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie (DGNC), 9. Joint Meeting mit der Japanischen Gesellschaft für Neurochirurgie. sine loco [digital], 21.-24.06.2020. Düsseldorf: German Medical Science GMS Publishing House; 2020. DocP131

doi: 10.3205/20dgnc416, urn:nbn:de:0183-20dgnc4167

Published: June 26, 2020

© 2020 Walter-Diessars et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution 4.0 License. See license information at http://creativecommons.org/licenses/by/4.0/.

Outline

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Objective: Ionizing radiation is an established risk factor for meningioma formation. However, the underlying mechanisms are largely unknown. It is discussed whether radiation triggers mutations or immunomodulatory processes, might lead to dedifferentiation of somatic cells and ultimately to the development of tumor stem cells. The aim of this study was to investigate the short-term effects of ionizing radiation on immunomodulatory processes in non-pathological dura mater cells (DMCs), which are known as the origin of meningioma development.

Methods: Three DMC cultures were cultivated under standard conditions. The confluent monolayers were exposed to a single dose of photon irradiation (1, 2, 4, 8, 12, 16, 20 Gy). 24h and 7, 14, 21 and 28 days after treatment, cells were passed and analyzed. Analyses were performed in respect to morphology (cell shape, generation times, etc.), molecular biology (qPCR), immunocytochemistry (ICC) and immunological aspects (ELISA) of tumor genesis.

Results: In the 1Gy and 8Gy radiation group (G) DMC morphology showed significant differences especially regarding the confluence and spherogenesis compared to the untreated group. Further correlations were found between the IL6 and IL8 levels in the 1GyG compared to the untreated group. We revealed 25% of NANOG positive tumor-like structures in the 1GyG versus 3% in the 8GyG by immunochemistry. A significantly higher NANOG gene expression was found in 1GyG on day 14 (first occurrence of spheroids) compared to 8GyG. Additionally, a tendency for CD126 expression in 1GyG compared to 8GyG could be found.

Conclusion: Based on the presented data, we concluded a correlation between the secretion of IL6 and IL8 and the formation of stem cell specific spheroids. Thus, the formation of a tumor stem cell-like phenotype seems to be possible as a short-term effect of radiation-induced cytokine secretion in the low-dose group.