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71. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie (DGNC)
9. Joint Meeting mit der Japanischen Gesellschaft für Neurochirurgie

Deutsche Gesellschaft für Neurochirurgie (DGNC) e. V.

21.06. - 24.06.2020

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Establishment of an in vivo adapted in vitro model of deep brain stimulation and investigations regarding the influence of electric stimulation on inflammatory mediators

Etablierung eines an die in vivoBedingungen angepasstenin vitroModells zur Untersuchung des Einflusses der elektrischen Stimulation auf inflammatorische Mediatoren

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  • presenting/speaker Henri Molkewehrum - Universitätsklinikum Schleswig-Holstein, Klinik für Neurochirurgie, Kiel, Deutschland
  • Carolin Kubelt - Universitätsklinikum Schleswig-Holstein, Klinik für Neurochirurgie, Kiel, Deutschland
  • Michael Synowitz - Universitätsklinikum Schleswig-Holstein, Klinik für Neurochirurgie, Kiel, Deutschland
  • Janka Held-Feindt - Universitätsklinikum Schleswig-Holstein, Klinik für Neurochirurgie, Kiel, Deutschland
  • Ann-Kristin Helmers - Universitätsklinikum Schleswig-Holstein, Klinik für Neurochirurgie, Kiel, Deutschland

Deutsche Gesellschaft für Neurochirurgie. 71. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie (DGNC), 9. Joint Meeting mit der Japanischen Gesellschaft für Neurochirurgie. sine loco [digital], 21.-24.06.2020. Düsseldorf: German Medical Science GMS Publishing House; 2020. DocP109

doi: 10.3205/20dgnc395, urn:nbn:de:0183-20dgnc3957

Published: June 26, 2020

© 2020 Molkewehrum et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution 4.0 License. See license information at http://creativecommons.org/licenses/by/4.0/.

Outline

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Objective: Deep brain simulation (DBS) is a neurosurgical therapy especially for movement disorders but also psychiatric diseases as depressions with a therapy-refractory course are rising. Even if the therapeutic results are mostly satisfactory, the mechanism of action is not completely understood. Possibly the effect of DBS is partly based on changed cytokine/ chemokine concentrations of the surrounding brain cells. Thus, in this study we established an in vivoadapted in vitromodel of DBS and analyzed the influence of an electric stimulation on different brain cells.

Methods: According to conditions used in DBS patients, we established an in vitromodel to electrically stimulate SVGA- (Astrocytes), HMC3- (Microglia) and SH-SY5Y-cells (Neurons). To exclude an influence on apoptosis or proliferation rates, cells were stained by TUNEL-Assay and proliferation rates were determined by cell counting compared to control samples, respectively. Additionally, we stimulated brain cells with 2mV for 24 hours and measured the expression of different cytokines and chemokines (CXCL12, CXCL16, CCL2, CCL20, IL1β, IL6) by qrtPCR and verified significant expression differences by fluorescence staining.

Results: The in vivoadapted in vitroDBS-model was established successfully. An influence on cell proliferation and apoptosis rates was not observed. mRNA expression of IL1βin SH-SY5Y-cells and CXCL12 in SVGA-cells were significantly induced, and these results were confirmed by fluorescence staining.

Conclusion: The mechanism of action of DBS seems to be very complex and its influence on the expression of cytokines and chemokines should be further explored. The establishment of this DBS-model could lead to a better understanding of these effects.