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71. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie (DGNC)
9. Joint Meeting mit der Japanischen Gesellschaft für Neurochirurgie

Deutsche Gesellschaft für Neurochirurgie (DGNC) e. V.

21.06. - 24.06.2020

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Metabolomic profiling with 1H NMR spectroscopy to predict disc degeneration in the human cervical spine

Vorhersagemöglichkeit der Degeneration der humanenHalswirbelsäule mittels Metabolom Analyse mit 1H-NMR-Spektroskopie

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  • Katharina Koch - Leibniz-Institut für umweltmedizinische Forschung (IUF), Leibniz Research Institute for Environmental Medicine, Düsseldorf, Deutschland
  • Marta Arrighi - Universitätsklinikum Düsseldorf, Neurochirurgische Klinik, Düsseldorf, Deutschland
  • Rudolf Hartmann - Forschungszentrum Jülich, Institute of Complex Systems (ICS), Jülich, Deutschland
  • Dieter Willbold - Forschungszentrum Jülich, Institute of Complex Systems (ICS), Jülich, Deutschland
  • Daniel Hänggi - Universitätsklinikum Düsseldorf, Neurochirurgische Klinik, Düsseldorf, Deutschland
  • Athanasios Petridis - Universitätsklinikum Düsseldorf, Neurochirurgische Klinik, Düsseldorf, Deutschland
  • Sajjad Muhammad - Universitätsklinikum Düsseldorf, Neurochirurgische Klinik, Düsseldorf, Deutschland
  • Ellen Fritsche - Leibniz-Institut für umweltmedizinische Forschung (IUF), Leibniz Research Institute for Environmental Medicine, Düsseldorf, Deutschland
  • presenting/speaker Richard Bostelmann - Universitätsklinikum Düsseldorf, Neurochirurgische Klinik, Düsseldorf, Deutschland

Deutsche Gesellschaft für Neurochirurgie. 71. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie (DGNC), 9. Joint Meeting mit der Japanischen Gesellschaft für Neurochirurgie. sine loco [digital], 21.-24.06.2020. Düsseldorf: German Medical Science GMS Publishing House; 2020. DocV012

doi: 10.3205/20dgnc012, urn:nbn:de:0183-20dgnc0123

Published: June 26, 2020

© 2020 Koch et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution 4.0 License. See license information at http://creativecommons.org/licenses/by/4.0/.

Outline

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Objective: Recently, imaging techniques providing metabolic representations of the tissue, such as magnetic resonance spectroscopy (MRS), are used to characterize disc degeneration. Only few studies focus on cervical intervertebral disc (IVD) degeneration. The purpose of this study is to identify promising biomarkers, detectable non-invasively by MRS in the patient, to provide novel in vivo imaging opportunities of discogenic pain in the human cervical spine.

Methods: Thirty biopsies of cervical pathological ID (Ranging-age: 20 -74 years) were dissected into annulus fibrosus (AF) and nucleus pulposus (NP). Water-soluble metabolites were extracted with the dual-phase method using methanol:chloroform:water (1:1:1). By 1H NMR spectroscopy the complete water-soluble metabolome was analysed on a HR 700 MHz spectrometer. Differences in metabolic composition of AP and NP were calculated and related to the degree of disc degeneration (I-V Miyazaki grading system, MGS). Significances were calculated with ANOVA analyses and Bonferroni’s multiple comparison tests.

Results: We detected changes in a variety of well-studied metabolites including lactate (Lac), choline (Cho), creatine (Cre), chondroitin (Chond), glutamine (Gln), glutamate (Glu), Tyrosine (Tyr), Glycine (Gly), and Succinate (Suc). We observed significantly (p < 0.0001) increased concentrations of Chond and significantly (p < 0.005) decreased concentrations of Cre, Gln, and Cho in NF compared to AF tissue. Strikingly, the difference in relative Chond between NP and AF was highest in Grade 3 and significantly (p-value < 0.05) decreased with increasing MGS (4 and 5). Moreover, we calculated several metabolic ratios and compared to MGS. Furthermore, we could detect a linear trend between the MGS and the extent of the observed differences in metabolite ratios (p < 0.05).

Conclusion: By means of assessing metabolic biomarkers with non-invasive 1H MRS spectroscopy, early stages of IVD degeneration could be detected. Especially the identified metabolic ratios such as Lac/Cre, Chon/Cre, Glu/Cre, or Cho/Cre, have potential for prediction of severity of the disc degeneration.