gms | German Medical Science

56. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie e. V. (DGNC)
3èmes journées françaises de Neurochirurgie (SFNC)

Deutsche Gesellschaft für Neurochirurgie e. V.
Société Française de Neurochirurgie

07. bis 11.05.2005, Strasbourg

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Inducible expression of p57KIP2 inhibits glioma cell motility and invasion

Die induzierbare p57KIP2 Expression hemmt die Zellmotilität und -invasion von Gliomzellen

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  • corresponding author A. Peraud - Department of Neurosurgery, Ludwig-Maximilians-University Munich, Munich
  • K. Sakai - The Arthur and Sonia Labatt Brain Tumour Research Centre, The University of Toronto, Toronto/CDN
  • T. Mainprize - The Arthur and Sonia Labatt Brain Tumour Research Centre, The University of Toronto, Toronto/CDN
  • J. Nakayama - The Arthur and Sonia Labatt Brain Tumour Research Centre, The University of Toronto, Toronto/CDN
  • A. Tsugu - The Arthur and Sonia Labatt Brain Tumour Research Centre, The University of Toronto, Toronto/CDN
  • K. Hongo - The Arthur and Sonia Labatt Brain Tumour Research Centre, The University of Toronto, Toronto/CDN
  • S. Kobayashi - The Arthur and Sonia Labatt Brain Tumour Research Centre, The University of Toronto, Toronto/CDN
  • J. T. Rutka - The Arthur and Sonia Labatt Brain Tumour Research Centre, The University of Toronto, Toronto/CDN

Deutsche Gesellschaft für Neurochirurgie. Société Française de Neurochirurgie. 56. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie e.V. (DGNC), 3èmes journées françaises de Neurochirurgie (SFNC). Strasbourg, 07.-11.05.2005. Düsseldorf, Köln: German Medical Science; 2005. DocP176

The electronic version of this article is the complete one and can be found online at: http://www.egms.de/en/meetings/dgnc2005/05dgnc0444.shtml

Published: May 4, 2005

© 2005 Peraud et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.en). You are free: to Share – to copy, distribute and transmit the work, provided the original author and source are credited.

Outline

Text

Objective

It has been hypothesized that migration and proliferation are mutually exclusive cellular programs in invasive gliomas. Previous results indicated that p57KIP2 overexpression, a cyclin-dependent kinase inhibitor (CDKI), significantly decreases cellular proliferation and promotes cell senescence in malignant glioma cell lines. The purpose of the present study was to focus on the migratory and invasive potential of malignant glioma cells under the influence of p57KIP2 expression, and to determine whether the above described dichotomous cellular behaviour holds for glioma cells expressing this CDKI.

Methods

Inducible stably transfected p57KIP2 expressing U373 and U87 glioma cell lines were used (in a tetracycline repressor system) to investigate differences in cell migration behaviour in a phagokinetic track assay on gold particles. Effects of extracellular matix (ECM) on U373 was determined in p57+ and p57- cells on surfaces coated with fibronectin, laminin, type I and IV collagens. Then the invasion of these cells across BD Biocoat Matrigel invasion chambers was determined.

Results

U373 motility was significantly reduced after p57KIP2 induction, and was not altered by different ECM proteins. The invasion chamber assay showed that p57+ cells exhibited a 35% reduction in their invasive capacity as compared to p57- cells.

Conclusions

Inducible expression of p57KIP2 in cell lines deficient in this CDKI reduces their motility and invasiveness. These results taken together with our previous ones demonstrate that p57KIP2 overexpression simultaneously downregulates proliferation and migration, which is in contrast to the initial hypothesis.

Forschungsstipendium der Deutschen Forschungsgemeinschaft (Pe 758/2-1)