gms | German Medical Science

55. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie e. V. (DGNC)
1. Joint Meeting mit der Ungarischen Gesellschaft für Neurochirurgie

Deutsche Gesellschaft für Neurochirurgie (DGNC) e. V.

25. bis 28.04.2004, Köln

Hypoxia Inducible Factor HIF-3 Gene Expression in Glioblastoma

Hypoxia-inducible-Factor-HIF-3-Genexpression in Glioblastomen

Meeting Abstract

  • corresponding author Kajetan von Eckardstein - Klinik für Neurochirurgie, HELIOS Klinikum Berlin, Klinikum Buch, Hobrechtsfelder Chaussee 96, 13125 Berlin
  • C. Schubert - Klinik für Neurochirurgie, HELIOS Klinikum Berlin, Klinikum Buch, Hobrechtsfelder Chaussee 96, 13125 Berlin
  • K. Dönneweg - Klinik für Neurochirurgie, HELIOS Klinikum Berlin, Klinikum Buch, Hobrechtsfelder Chaussee 96, 13125 Berlin
  • R. Reszka - Klinik für Neurochirurgie, HELIOS Klinikum Berlin, Klinikum Buch, Hobrechtsfelder Chaussee 96, 13125 Berlin
  • J. Kiwit - Klinik für Neurochirurgie, HELIOS Klinikum Berlin, Klinikum Buch, Hobrechtsfelder Chaussee 96, 13125 Berlin

Deutsche Gesellschaft für Neurochirurgie. Ungarische Gesellschaft für Neurochirurgie. 55. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie e.V. (DGNC), 1. Joint Meeting mit der Ungarischen Gesellschaft für Neurochirurgie. Köln, 25.-28.04.2004. Düsseldorf, Köln: German Medical Science; 2004. DocP 05.45

The electronic version of this article is the complete one and can be found online at: http://www.egms.de/en/meetings/dgnc2004/04dgnc0328.shtml

Published: April 23, 2004

© 2004 von Eckardstein et al.
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Outline

Text

Objective

Primary and secondary gliobastomas are characterized by different genetic alterations. In order to identify new tumor-associated genes, glioblastoma probes were analyzed using "Human Unigene Set II" filters containing 72.000 cDNA clones representing genes of all human organs.

Methods

RNA was extracted from 74 tumor probes including low-grade astrocytomas and primary and secondary glioblastomas, reverse transcribed into cDNA and hybridized to cDNA filters.

Results

In comparison to regular brain tissue, we identified the hypoxia-inducible factor HIF-3 alpha being overexpressed in 40% of all glioblastomas (23/56) and in 30% of anaplastic astrocytoma (4/14). Samples from low-grade glioma did not show any overexpression.

Conclusions

HIF-3 transcription factor induces gene expression by binding to a hypoxia-responsive element. Enhanced genes might be involved in angiogenesis. It is known that HIF-1 alpha is upregulated in about 70% of glioblastomas. Our findings indicate that HIF-3 is also involved in the neovascularisation of high-grade astrocytoma in response to hypoxia.