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55. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie e. V. (DGNC)
1. Joint Meeting mit der Ungarischen Gesellschaft für Neurochirurgie

Deutsche Gesellschaft für Neurochirurgie (DGNC) e. V.

25. bis 28.04.2004, Köln

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Differential gene expression in adult neural progenitor cells from the human dentate gyrus and olfactory bulb

Differentielle Genexpression in adulten neuralen Vorläuferzellen aus dem humanen Gyrus dentatus und Bulbus olfactorius

Meeting Abstract

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  • corresponding author Christine Dictus - Neurochirurgische Universitätsklinik Heidelberg, Heidelberg
  • K. Steiner - Forschungsschwerpunkt Molekulare Genomanalyse, Deutsches Krebsforschungszentrum, Heidelberg
  • H. H. Steiner - Neurochirurgische Universitätsklinik Heidelberg, Heidelberg
  • V. Tronnier - Neurochirurgische Universitätsklinik Heidelberg, Heidelberg
  • C. Herold-Mende - Neurochirurgische Universitätsklinik Heidelberg, Heidelberg

Deutsche Gesellschaft für Neurochirurgie. Ungarische Gesellschaft für Neurochirurgie. 55. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie e.V. (DGNC), 1. Joint Meeting mit der Ungarischen Gesellschaft für Neurochirurgie. Köln, 25.-28.04.2004. Düsseldorf, Köln: German Medical Science; 2004. DocP 03.30

The electronic version of this article is the complete one and can be found online at: http://www.egms.de/en/meetings/dgnc2004/04dgnc0313.shtml

Published: April 23, 2004

© 2004 Dictus et al.
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Outline

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Objective

In the last few years, several neurogenic regions have been identified in the adult brain of rodents and humans, of those being most important the dentate gyrus (DG) belonging to the hippocampal region and the so-called rostral migratory pathway leading from the subventricular zone of the lateral ventricles to the olfactory bulb (BO). However, little is known about the differential characteristics of neural progenitor cells coming from either one of these two major neurogenic regions. Therefore the aim of the present study was to identify genes being differentially expressed in the dentate gyrus and the olfactory bulb of the adult human brain, by using mRNA microarray analysis.

Methods

Neural progenitor cell cultures were established from the dentate gyrus and the olfactory bulb of adult patients undergoing neurosurgical operations. Expression of various differentiation markers was demonstrated by immunohistochemistry and RT-PCR. Furthermore, RNA of early and advanced passages was subjected to mRNA microarray analysis.

Results

Primary cultures of adult neural progenitor cells isolated from the human dentate gyrus (DG) and the human olfactory bulb (BO) expressed the neural stem cell marker nestin as well as markers of the neuronal and glial cell lineage on protein and on mRNA level. Although these cells showed a similar expression of the markers analysed, mRNA microarray analysis comparing DG cells and BO cells revealed differential expression of a number of genes. Most of them are associated with a high proliferation capacity and, interestingly, were overexpressed in the DG cell lineage.

Conclusions

Our data suggest that adult neural progenitor cells from the dentate gyrus might have a higher proliferation potential. This can be of importance for future therapeutic applications.