Article
A model for intratumoural chemotherapy in the rat brain
Implantationsmodell für intratumorale Chemotherapie bei experimentellen Rattenhirntumoren
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Authors
Published: | April 23, 2004 |
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Outline
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Objective
To achieve the best reproducibility in rat brain tumour models, several injection techniques are currently in use. Although stereotactical cells injection has proved to be effective and reliable, it is expensive and time consuming. A new permanently implanted device is presented here. It allows precise cell delivery for best tumour reproducibility, and can be left in place for future injections - at exactly the same location - such as intratumoural chemotherapy.
Methods
A Teflon tube was mounted on a disk, inserted into the rat brain and sealed to the skull. The device was tested in two rat strains (Wistar and New Zealand Nude rats) with two different glioma cell lines (9L and C6). Rats were treated with placebo to determine if repeated treatments had an effect on the device placement, or if device-related morbidity was induced.
Results
Analysis of brain sections showed that the device path was always within the tumour. The device never moved or came off the scalp. Both Wistar rats and NZ nude rats tolerated the device well. No morbidity or mortality was observed, regardless of the presence of the device; no infections were seen.
Conclusions
Biocompatible, non-irritating and well tolerated; such a device can be used for reproducible tumour cell injection and repeated intralesional delivery of drugs.