gms | German Medical Science

55. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie e. V. (DGNC)
1. Joint Meeting mit der Ungarischen Gesellschaft für Neurochirurgie

Deutsche Gesellschaft für Neurochirurgie (DGNC) e. V.

25. bis 28.04.2004, Köln

A model for intratumoural chemotherapy in the rat brain

Implantationsmodell für intratumorale Chemotherapie bei experimentellen Rattenhirntumoren

Meeting Abstract

  • corresponding author Mattia Bellinzona - Neurochirurgische Klinik, Klinikum Hannover Nordstadt, Hannover
  • F. Roser - Neurochirurgische Klinik, Klinikum Hannover Nordstadt, Hannover
  • M. Saini - Neurochirurgische Klinik, Klinikum Hannover Nordstadt, Hannover
  • M. Samii - International Neusorscience Institute, Hannover

Deutsche Gesellschaft für Neurochirurgie. Ungarische Gesellschaft für Neurochirurgie. 55. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie e.V. (DGNC), 1. Joint Meeting mit der Ungarischen Gesellschaft für Neurochirurgie. Köln, 25.-28.04.2004. Düsseldorf, Köln: German Medical Science; 2004. DocMO.12.01

The electronic version of this article is the complete one and can be found online at: http://www.egms.de/en/meetings/dgnc2004/04dgnc0116.shtml

Published: April 23, 2004

© 2004 Bellinzona et al.
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Outline

Text

Objective

To achieve the best reproducibility in rat brain tumour models, several injection techniques are currently in use. Although stereotactical cells injection has proved to be effective and reliable, it is expensive and time consuming. A new permanently implanted device is presented here. It allows precise cell delivery for best tumour reproducibility, and can be left in place for future injections - at exactly the same location - such as intratumoural chemotherapy.

Methods

A Teflon tube was mounted on a disk, inserted into the rat brain and sealed to the skull. The device was tested in two rat strains (Wistar and New Zealand Nude rats) with two different glioma cell lines (9L and C6). Rats were treated with placebo to determine if repeated treatments had an effect on the device placement, or if device-related morbidity was induced.

Results

Analysis of brain sections showed that the device path was always within the tumour. The device never moved or came off the scalp. Both Wistar rats and NZ nude rats tolerated the device well. No morbidity or mortality was observed, regardless of the presence of the device; no infections were seen.

Conclusions

Biocompatible, non-irritating and well tolerated; such a device can be used for reproducible tumour cell injection and repeated intralesional delivery of drugs.