gms | German Medical Science

132. Kongress der Deutschen Gesellschaft für Chirurgie

Deutsche Gesellschaft für Chirurgie

28.04. - 01.05.2015, München

Do B cells and humoral immunity contribute to experimental bronchiolitis obliterans syndrome?

Meeting Abstract

  • Martin Reichert - Labor für Experimentelle Chirurgie, Klinik für Allgemein- und Thoraxchirurgie, Gießen, Deutschland
  • Srebrena Atanasova - Labor für Experimentelle Chirurgie, Klinik für Allgemein- und Thoraxchirurgie, Gießen, Deutschland
  • Gabriele Fuchs-Moll - Labor für Experimentelle Chirurgie, Klinik für Allgemein- und Thoraxchirurgie, Gießen, Deutschland
  • Kathrin Petri - Labor für Experimentelle Chirurgie, Klinik für Allgemein- und Thoraxchirurgie, Gießen, Deutschland
  • Winfried Padberg - Labor für Experimentelle Chirurgie, Klinik für Allgemein- und Thoraxchirurgie, Gießen, Deutschland
  • Veronika Grau - Universitätsklinikum Gießen, Klinik für Allgemein-, Viszeral-, Thorax-, Transplantations- und Kinderchirurgie, Gießen, Deutschland

Deutsche Gesellschaft für Chirurgie. 132. Kongress der Deutschen Gesellschaft für Chirurgie. München, 28.04.-01.05.2015. Düsseldorf: German Medical Science GMS Publishing House; 2015. Doc15dgch634

doi: 10.3205/15dgch634, urn:nbn:de:0183-15dgch6346

Published: April 24, 2015

© 2015 Reichert et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution 4.0 License. See license information at http://creativecommons.org/licenses/by/4.0/.


Outline

Text

Introduction: Recently, we established a clinically relevant experimental model for human bronchiolitis obliterans syndrome (BOS), which involves orthotopic transplantation of rat lung allografts followed by intratracheal application of lipopolysaccharide (LPS) (Atanasova et al. 2013, J Heart Lung Transplant 32: 1131). Chronic lung damage does neither develop in allografts treated with vehicle instead of LPS nor in pulmonary isografts. Alloreactive and autoreactive antibodies have been detected in BOS patients but their pathogenic role is disputed. Here, we investigate B cell infiltration into experimental lung allografts as well as the deposition of immunoglobulins and C4d.

Material and methods: Orthotopic left lung transplantation was performed in the Fischer-344 to Lewis strain combination followed by application of ciclosporine (5 mg/kg) for 10 days. Lewis rats served as isograft recipients. Four weeks after transplantation, LPS (0.5 mg/kg body weight) was instilled into the trachea. Lungs were harvested before (day 28) and after LPS application (days 29, 33, and 40) for immunohistochemistry.

Results: Perivascular and peribronchiolar areas of lung allografts were more strongly infiltrated by B cells in comparison to right native lungs and isografts. Interestingly, an influx of B cells into the alveolar region was induced in response to LPS application only in allografts. Immunoglobulin-positive cells were markedly increased in the alveolar space of lung allografts compared to isografts at days 33 and 40. C4d deposits were mainly found in the wall of small blood vessels as well as on the respiratory epithelium of lung allografts.

Conclusion: These results suggest that B cells play a role in the development of BOS by producing antibodies against donor tissue. As a marker for antibody mediated rejection in other solid organ transplantation, deposition of C4d could be a prognostic factor for lung allograft survival.