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127. Kongress der Deutschen Gesellschaft für Chirurgie

Deutsche Gesellschaft für Chirurgie

20.04. - 23.04.2010, Berlin

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The effect of chronic and acute wound fluids on human keratinocytes and adipose-derived stem cells

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  • Timo Spanholtz - Universität Witten/Herdecke, Campus Köln-Merheim, Plastische und Rekonstruktive Chirurgie, Köln, Deutschland
  • Oliver Thamm - Universität Witten/Herdecke, Campus Köln-Merheim, Plastische und Rekonstruktive Chirurgie, Köln, Deutschland
  • Vu Phan - Universität Witten/Herdecke, Campus Köln-Merheim, Plastische und Rekonstruktive Chirurgie, Köln, Deutschland
  • Marc Maegele - Universität Witten/Herdecke, Campus Köln-Merheim, Department of Trauma and Orthopedic Surgery, Köln, Deutschland
  • Alina Schneider - Universität Witten/Herdecke, Campus Köln-Merheim, Plastische und Rekonstruktive Chirurgie, Köln, Deutschland
  • Nicola Bader - Universität Witten/Herdecke, Campus Köln-Merheim, Plastische und Rekonstruktive Chirurgie, Köln, Deutschland
  • Edmund A.M. Neugebauer - Universität Witten/Herdecke, Campus Köln-Merheim, Institut für Forschung in der Operativen Medizin (IFOM), Köln, Deutschland

Deutsche Gesellschaft für Chirurgie. 127. Kongress der Deutschen Gesellschaft für Chirurgie. Berlin, 20.-23.04.2010. Düsseldorf: German Medical Science GMS Publishing House; 2010. Doc10dgch171

doi: 10.3205/10dgch171, urn:nbn:de:0183-10dgch1717

Published: May 17, 2010

© 2010 Spanholtz et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.en). You are free: to Share – to copy, distribute and transmit the work, provided the original author and source are credited.

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Introduction: Although chronic wounds are common and represent a growing social-economic problem, treatment for this disabling condition remains limited and largely ineffective. Optimum healing requires a well-orchestrated interaction of growth factors and keratinocytes (KC). It is generally believed that their function is impaired by factors from chronic wound environment. Stimulating factors derived from applied adipose-derived stem cells (ASC) may reactivate keratinocyte function. Aim of this study was to evaluate the effects of chronic and acute wound fluids on ASC and KC behaviour.

Materials and methods: After informed consent we harvested adipose-derived stem cells and keratinocytes from healthy human donors and extracted chronic and acute wound fluids from standardised patients´ wounds. ASC and KC were incubated with CWF and AWF and proliferation, migration and protein / gen expression patterns were measured. Further, wound-healing competence of KC was measured in an in vitro wound-healing model.

Results: ASC and KC could be cultured and characterised. Amount of overall protein in CWF and AWF was comparable (27,27 vs. 31,73mg/ml). Under the influence of AWF and CWF both cell types showed changes in proliferation and migration behaviour. Gen/protein analysis showed changes in VEGF, FGF2, FGF7 as well as MMP2 and MMP9 expression in both cell lines. The competence of KC to close a defined wound in vitro was impaired by CWF, which on the other hand stimulated the migration rate of ASC in vitro.

Conclusion: The fluids from chronic and acute wounds affect the behaviour of human ASC and KC in vitro. The subsequent decrease of KC migration and proliferation might be responsible for delayed wound healing in chronic wounds. ASC are not negatively influenced by CWF or AWF. In the future, growth factors expressed by transplanted ASC can possibly re-activate impaired KC function.