gms | German Medical Science

Objective: In this project we evaluate the efficacy of advanced multifunctional microelectrode array (MEA) devices in order to improve our understanding of brain dynamics and optimize pharmaceutical approaches for neurological disorders.

Materials and Methods: Electrophysiological recordings were made using the Multi-Channel Systems GmbH ME2100 with custom-made Flex-MEAs holding 128 microelectrodes (Flex-MEA 128) [1]. Brain slices from mice and humans were kept at 37° C with 5% CO₂. The low-impedance Flex-MEA 128 offers excellent signal-to-noise recordings. Perforations allow gases and nutrients to perfuse brain slices for longer periods. The Flex-MEA 128 is connected to a custom printed circuit board and enclosed in a metal case to reduce noise. It is linked to µPA-32 headstages of the ME 2100 for data collection. Over 24 hours recordings were made sequentially. Chemicals as neuro-stimulants and -inhibitors were added to brain slices to better understand the signaling. Future studies will examine microfluidic MEAs for precise neurotransmitter of drug delivery. Combined patch-clamp and CMOS recordings will be used for neural modulation and monitoring of pharmaceutical effects [2].

Results: Successful long-term continuous electrophysiological recordings in real time with murine and human slices were achieved by Flex-MEA 128. Long-term recordings over multiple days in a configuration that mimics the somatic system offer a promising tool for future pharmacological research.

Discussion: We will study in-vitro drug effects on neural tissues with the non-invasive Flex-MEA 128 system utilizing electrical and biochemical stimulation