gms | German Medical Science

GMS Current Posters in Otorhinolaryngology - Head and Neck Surgery

German Society of Oto-Rhino-Laryngology, Head and Neck Surgery (DGHNOKHC)

ISSN 1865-1038

Could fungi be detected in the fluid of persistent otitis media with effusion?

Poster Otologie

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  • corresponding author Mohamed Eid - Fayoum university, Fayoum, Egypt
  • Waleed Jabri - Fayoum University, Faoym, Eygpt
  • Wael Wageh - Fayoum University, Faoym, Eygpt
  • Mostafa Ezzat - Fayoum University, Faoym, Eygpt

GMS Curr Posters Otorhinolaryngol Head Neck Surg 2017;13:Doc169

doi: 10.3205/cpo001723, urn:nbn:de:0183-cpo0017231

Published: April 26, 2017

© 2017 Eid et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution 4.0 License. See license information at



Background: Otitis media with effusion (OME) often is considered a direct extension of the inflammatory process that occurs during long-lasting or recurrent episodes of acute otitis media. Most bacterial and viral cultures of middle ear fluid that had been performed were often negative suggesting that other infectious agents may be involved such as fungi.

Materials and methods: Thirty patients suffering from chronic secretory otitis media (OME) were enrolled. Three samples were collected and investigated using PCR assay with universal fungal primers and Sabouraud agar. The first sample was obtained from the fluid of the middle ear before insertion of the ventilation tube; the second sample was obtained from nasal secretions; and the third sample was obtained from the ipsilateral peritubal area of the nasopharynx. Samples were tested using PCR assay with universal fungal primers and Sabouraud agar.

Results: PCR examination of the middle ear aspirate in group cases was positive in 7 cases (23.3%), in nasal secretions samples 2 cases only (13.3%) were positive and no positive cases were detected in nasopharyngeal swab samples. Sabouraud agar culture was positive for fungal culture of middle ear aspirates in 5 cases (16.6%) but in no cases for nasal secretion samples. Results also showed negative(N0) growth in 30 (100%) patients for nasopharyngeal swab on Sabouraud agar.

Conclusion: Fungal DNA could be detected in the middle ear fluid in seven (23.3%) of 30 patients with persistent OME using PCR assay and fungi could be detected in five (16.6%) patients on Sabouraud agar. A significant relationship was found between detection of fungi in the middle ear fluid and the duration of the disease, associated adenoid, and history of asthma.

Supported by: Otolaryngology Department Fayoum University, Clinical Pathology Department Fayoum University, Clinical Pathology Department National Institute OF Oncology

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