Artikel
Synthetic peptide studies on the SARS coronavirus spike Ggycoprotein: Perspective for SARS vaccine development
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Veröffentlicht: | 26. Mai 2004 |
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Background: The S (spike) protein of the etiologic coronavirus agent of the severe acute respiratory syndrome (SARS) plays a central role in mediating viral infection via receptor binding and membrane fusion between the virion and the host cell. We focused on using synthetic peptides for developing antibodies against SARS-CoV, which aimed to block viral invasion by eliciting an immune response specific to the native SARS-CoV S protein.
Methods: Six peptide sequences corresponding to the surface regions of SARS-CoV S protein were designed and investigated using combined bioinformatics and structural analysis. These synthetic peptides were used to immunize both rabbits and monkeys. Antisera collected one week after the second immunization were analyzed by ELISA and tested for antibody specificity against SARS-CoV by immunofluorescent confocal microscopy.
Results: Four of our six synthetic peptides (namely S2, S3, S5 and S6) elicited SARS-CoV-specific antibodies, from which S5 (residues 788-820) and S6 (residues 1002-1030) exhibited similar immunogenic responses as compared with a parallel investigation employing truncated recombinant protein analogs of the SARS-CoV S protein. This suggested that our S5 and S6 peptides may represent two minimum biologically active sequences of the immunogenic regions of the SARS-CoV S protein.
Conclusions: Synthetic peptides can elicit specific antibodies to SARS-CoV. The study provides insights for the future development of SARS vaccine via the synthetic peptide-based approach.
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