gms | German Medical Science

12th Malaria Meeting

Malaria Group / Section Antiparasitic Chemotherapy of the Paul-Ehrlich-Society (PEG e. V.) in cooperation with the German Society for Tropical Medicine and International Health (DTG e. V.) and the German Society for Parasitology (DGP e. V.)

14.11. - 15.11.2014, Bonn

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Characterization of a plant-derived malaria vaccine candidate based on a Plasmodium falciparum sexual-stage fusion protein

Meeting Abstract

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  • Veronique Beiss - Department Plant Biotechnology, Fraunhofer Institute for Molecular Biology and Applied Ecology (IME), Aachen, Germany
  • Alexander Boes - Department Plant Biotechnology, Fraunhofer Institute for Molecular Biology and Applied Ecology (IME), Aachen, Germany
  • Holger Spiegel - Department Plant Biotechnology, Fraunhofer Institute for Molecular Biology and Applied Ecology (IME), Aachen, Germany
  • Stephanie Kapelski - Department Plant Biotechnology, Fraunhofer Institute for Molecular Biology and Applied Ecology (IME), Aachen, Germany
  • Matthias Scheuermayer - Research Center for Infectious Diseases, University of Wuerzburg, Wuerzburg, Germany
  • Güven Edgue - Department Plant Biotechnology, Fraunhofer Institute for Molecular Biology and Applied Ecology (IME), Aachen, Germany
  • Markus Sack - Institute for Molecular Biotechnology, RWTH Aachen University, Aachen, Germany
  • Andreas Reimann - Department Plant Biotechnology, Fraunhofer Institute for Molecular Biology and Applied Ecology (IME), Aachen, Germany
  • Stefan Schillberg - Department Plant Biotechnology, Fraunhofer Institute for Molecular Biology and Applied Ecology (IME), Aachen, Germany
  • Gabriele Pradel - Institute for Molecular Biotechnology, RWTH Aachen University, Aachen, Germany
  • Rainer Fischer - Department Plant Biotechnology, Fraunhofer Institute for Molecular Biology and Applied Ecology (IME), Aachen, Germany; Institute for Molecular Biotechnology, RWTH Aachen University, Aachen, Germany

12th Malaria Meeting. Bonn, 14.-15.11.2014. Düsseldorf: German Medical Science GMS Publishing House; 2014. Doc14mal13

doi: 10.3205/14mal13, urn:nbn:de:0183-14mal131

Veröffentlicht: 17. Dezember 2014

© 2014 Beiss et al.
Dieser Artikel ist ein Open Access-Artikel und steht unter den Creative Commons Lizenzbedingungen (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.de). Er darf vervielfältigt, verbreitet und öffentlich zugänglich gemacht werden, vorausgesetzt dass Autor und Quelle genannt werden.

Gliederung

Text

Plants provide a low-cost production platform for vaccines targeting poverty-related diseases such as malaria, although the feasibility of production depends on the functional efficacy, stability, yield and purification of the vaccine. Here we describe the high-level production and functional characterization of a new plant-derived malaria vaccine candidate targeting the sexual stage of Plasmodium falciparum. The fusion protein F0 (Pfs25 and Pfs230_C0) was transiently expressed in Nicotiana benthamiana leaves by agroinfiltration. The protein accumulated to 9% TSP, was thermostable up to 80°C and could be purified to >90% using a simple two step procedure. Analysis of the mouse sera generated by immunization revealed a good immune response against the full length protein F0 and a moderate response against the individual antigens. The immunogenic conformation of the purified F0 protein was indicated by sera reactivity from semi-immune donors. Immunofluorescence assays showed that the total IgG from the mouse sera recognized several P. falciparum development stages specifically, and the sera induced up to 100% transmission-blocking activity. These results underline the potential of plant derived F0 as a novel sexual stage vaccine candidate offering the advantages of high-level accumulation and thermostability.