gms | German Medical Science

14th Triennial Congress of the International Federation of Societies for Surgery of the Hand (IFSSH), 11th Triennial Congress of the International Federation of Societies for Hand Therapy (IFSHT)

17.06. - 21.06.2019, Berlin

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The effects of Adipose derived stem cell on optimizing Achilles tendon repair in rat

Meeting Abstract

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  • presenting/speaker Mohammad Javad Fatemi - Burn Research Center, Iran University of Medical Sciences, Tehran, Iran
  • Fatemeh Khodaei - Burn Research Center, Iran University of Medical Sciences, Tehran, Iran
  • Hossein Salehi - Burn Research Center, Iran University of Medical Sciences, Tehran, Iran
  • Tooran Bagheri - Burn Research Center, Iran University of Medical Sciences, Tehran, Iran
  • Mitra Niazi - Burn Research Center, Iran University of Medical Sciences, Tehran, Iran
  • Seyed Aboozar Hosseini - Burn Research Center, Iran University of Medical Sciences, Tehran, Iran

International Federation of Societies for Surgery of the Hand. International Federation of Societies for Hand Therapy. 14th Triennial Congress of the International Federation of Societies for Surgery of the Hand (IFSSH), 11th Triennial Congress of the International Federation of Societies for Hand Therapy (IFSHT), 11th Triennial Congress of the International Federation of Societies for Hand Therapy (IFSHT). Berlin, 17.-21.06.2019. Düsseldorf: German Medical Science GMS Publishing House; 2020. DocIFSSH19-313

doi: 10.3205/19ifssh1195, urn:nbn:de:0183-19ifssh11957

Veröffentlicht: 6. Februar 2020

© 2020 Fatemi et al.
Dieser Artikel ist ein Open-Access-Artikel und steht unter den Lizenzbedingungen der Creative Commons Attribution 4.0 License (Namensnennung). Lizenz-Angaben siehe http://creativecommons.org/licenses/by/4.0/.

Gliederung

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Objectives/Interrogation: Most important factor in having more favorable outcome is strength of repaired tendon and lack of adherence to the adjacent tissues. Various methods have been proposed to improve the process of repair and prevent adhesion. The aim of this study is to survey the effects of adipose derived stem cells on optimizing Achilles tendon repair in rat.

Methods: In this research 36 Sprague-Dawley male rats weighing approximately 250 to 300 grams, were randomly divided into three groups. Autologous fat derived stem cells were prepared from inguinal fat pad of 12 rats. Achilles tendon was cut in all 3 groups. Autologous stem cell was injected into the both cut ends of tendon before repair in the first group. In the second group normal saline and in the third group culture medium without cell was injected in the cut ends of the tendons. Strength and quality of repair were surveyed by tensometry and histopathology evaluation after 30 days of repair.

Results and Conclusions: Results showed that the group of stem cell had the most tendon strength with statistically significant difference to other groups (p-value=0.017). The histopathology indices such as the amount of collagen, fibroblasts and else were not significantly different. Although, metaplasia was more common in culture and normal saline groups than stem cell, but the difference was not significant (p-value= 0.02). This study showed that stem cells could improve biomechanics of tendon repair without any pathologic changes. Although, it was unexpected to have metaplasia in control and culture groups which necessitates more studies in this field.