gms | German Medical Science

14th Triennial Congress of the International Federation of Societies for Surgery of the Hand (IFSSH), 11th Triennial Congress of the International Federation of Societies for Hand Therapy (IFSHT)

17.06. - 21.06.2019, Berlin

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Validation of a Novel Rabbit Model of Compression Neuropathy in the Setting of Perineural Adhesion

Meeting Abstract

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  • presenting/speaker Steven Koehler - SUNY Downstate Medical Center, Department of Orthopaedic Surgery, Hand and Microsurgery, Brooklyn, United States
  • Neil Shah - SUNY Downstate Medical Center, Department of Orthopaedic Surgery, Hand and Microsurgery, Brooklyn, United States
  • Westley Hayes - SUNY Downstate Medical Center, Department of Orthopaedic Surgery, Hand and Microsurgery, Brooklyn, United States
  • William Chin - SUNY Downstate Medical Center, Department of Orthopaedic Surgery, Hand and Microsurgery, Brooklyn, United States
  • John Carter - SUNY Downstate Medical Center, Department of Orthopaedic Surgery, Hand and Microsurgery, Brooklyn, United States
  • Adriano Martins - SUNY Downstate Medical Center, Department of Orthopaedic Surgery, Hand and Microsurgery, Brooklyn, United States

International Federation of Societies for Surgery of the Hand. International Federation of Societies for Hand Therapy. 14th Triennial Congress of the International Federation of Societies for Surgery of the Hand (IFSSH), 11th Triennial Congress of the International Federation of Societies for Hand Therapy (IFSHT), 11th Triennial Congress of the International Federation of Societies for Hand Therapy (IFSHT). Berlin, 17.-21.06.2019. Düsseldorf: German Medical Science GMS Publishing House; 2020. DocIFSSH19-1558

doi: 10.3205/19ifssh1186, urn:nbn:de:0183-19ifssh11860

Veröffentlicht: 6. Februar 2020

© 2020 Koehler et al.
Dieser Artikel ist ein Open-Access-Artikel und steht unter den Lizenzbedingungen der Creative Commons Attribution 4.0 License (Namensnennung). Lizenz-Angaben siehe http://creativecommons.org/licenses/by/4.0/.

Gliederung

Text

Objectives/Interrogation: Nerve injury initiates an inflammatory cascade that results in nerve scarring, fibrosis, and adhesions, leading to development of compression neuropathy. Rabbit nerve models more closely mimic human biology. This study sought to develop a novel, rabbit model to simulate compression neuropathy secondary to perineural adhesion.

Methods: Sciatic nerves of five adult, 3-4 kg New Zealand White Rabbits were surgically-exposed. Left hindlimbs underwent sham surgery. Perineural adhesions were generated via bipolar electrocautery on 10 mm of the nerve bed, and the epineurium was fixed with microsuture to the cauterized wound bed (right). Rabbits were maintained for six weeks. Exposure of the sciatic nerve was performed bilaterally, with proximal and distal nerve transection, at which point peak pull-out force (N) required to break adhesions via pulley-mounting on a uniaxial biomechanical testing frame was measured (Figure 1 [Fig. 1]). Peak pull-out force was compared using unpaired Wilcoxons rank sum test. Tibialis anterior (TA) muscles were harvested bilaterally, and muscle mass (grams) was compared between hindlimbs.

Results and Conclusions: Scarred nerves required greater peak pull-out force than control nerves (2.51 N vs. 0.50 N, p=0.021) (Figure 2 [Fig. 2]). TA muscle mass was significantly lower in the setting of induced neuropathy when compared to sham surgery (6.56 g vs. 8.52 g, p<0.001). We developed and validated a model to simulate compression neuropathy. Additionally, we created a novel means to measure peak pull-force required to break perineural adhesion as a quantitative surrogate for perineural scar. This model will provide surgeons a validated, animal model to pursue translational study of compression neuropathy.