gms | German Medical Science

18th Symposium on Infections in the Immunocompromised Host

International Immunocompromised Host Society

15. to 17.06.2014, Berlin

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Diversity of C Subgenotype of HHV-8 found in the Saliva of HIV-Infected Individuals Without KS Under the Use of HAART in Brazil

Meeting Abstract

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  • P.H. Braz-Silva - University of São Paulo, São Paulo, Brazil
  • T.R. Tozetto Mendoza - Brazil
  • L.M. Sumita - Brazil
  • W.S. Freire - Brazil
  • V.U.F. de Souza - Brazil
  • C.S. Pannuti - Brazil

18th Symposium on Infections in the Immunocompromised Host. Berlin, 15.-17.06.2014. Düsseldorf: German Medical Science GMS Publishing House; 2014. Doc14ichs37

doi: 10.3205/14ichs37, urn:nbn:de:0183-14ichs379

Veröffentlicht: 3. Juni 2014

© 2014 Braz-Silva et al.
Dieser Artikel ist ein Open Access-Artikel und steht unter den Creative Commons Lizenzbedingungen (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.de). Er darf vervielfältigt, verbreitet und öffentlich zugänglich gemacht werden, vorausgesetzt dass Autor und Quelle genannt werden.

Gliederung

Text

Few studies have addressed the identification of HHV-8 genotypes detected in saliva in the absence of KS. Our study aimed to identify the genotypes of HHV-8 in saliva of HIV-infected Brazilians under HAART. We assayed saliva samples of 751 individuals for HHV-8 viral load and for the detection of DNA/HHV-8 fragments by nested PCR of the ORF K1 and subsequent genotyping by ABI platform. The HHV-8 viral load was detected in 106/751 (14.1%), ranging from 4 copies to 6 billion copies/µg of DNA. We obtained 33/106 (31.1%) ORF K1 sequences of 400 to 800 bp of the genotypes A1, A2, B1, B2, C1, C2, C3, C5, C7 and F. Additionally, 12.2% of samples were identified as subtype A and 35.3% as B and C. We found 9% of K1 recombinant genomes. However, the diversity of genotype C was striking in this group presenting previously identified circulating recombinant forms.