gms | German Medical Science

87. Jahresversammlung der Deutschen Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie e. V.

Deutsche Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie e. V.

04.05. - 07.05.2016, Düsseldorf

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Neurite Outgrowth of Human Induced Pluripotent Stem Cell-derived Neurons – examination of the effect of different factors

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  • corresponding author Desislava Skerleva - Department of Otolaryngology, Head and Neck Surgery, Kyoto University, Kyoto, Japan
  • Hiroe Ohnishi - Department of Otolaryngology, Head and Neck Surgery, Kyoto University, Kyoto, Japan
  • Stefan Stoyanov - Medical Institute Ministry of Interior, Sofia, Bulgaria
  • Norio Yamamoto - Department of Otolaryngology, Head and Neck Surgery, Kyoto University, Kyoto, Japan
  • Juichi Ito - Hearing Communication Medical Center, Shiga Medical Center Research Institute, Shiga, Japan
  • Takayuki Nakagawa - Department of Otolaryngology, Head and Neck Surgery, Kyoto University, Kyoto, Japan

Deutsche Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie. 87. Jahresversammlung der Deutschen Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie. Düsseldorf, 04.-07.05.2016. Düsseldorf: German Medical Science GMS Publishing House; 2016. Doc16hnod635

doi: 10.3205/16hnod635, urn:nbn:de:0183-16hnod6359

Veröffentlicht: 30. März 2016

© 2016 Skerleva et al.
Dieser Artikel ist ein Open-Access-Artikel und steht unter den Lizenzbedingungen der Creative Commons Attribution 4.0 License (Namensnennung). Lizenz-Angaben siehe http://creativecommons.org/licenses/by/4.0/.

Gliederung

Text

Introduction: Induced pluripotent stem cells allow us to confirm the results from animal experiments on human cells in a safe and convenient way. This give us the opportunity to investigate their role in the field of regenerative medicine for inner ear diseases.

Methods: The effect of BDNF, NT-3 and IGF-1 on neurite outgrowth of human induced pluripotent stem (hiPS) cell-derived neurons was examined. First, human iPS cell line 201B7-GFP was differentiated into neural progenitor cells. Neurospheres were prepared from neural progenitor cells by floating culture on U-bottom low adhesion plate. After that the neurospheres were transferred on Matrigel-coated plates and underwent neuronal differentiation for 7 days with or without additional neurotrophic or growth factors.

Results: Immunocytochemistry showed βIII-tubulin and neurofilament positive cells in the neurospheres. The neurites of each neurosphere were counted. No significant difference was observed between the different groups.

Conclusions: Previous studies demonstrate the effect of BDNF, NT-3 and IGF-1 on neurite outgrowth. In our study for the first time this assessment is done by using human iPS cells. Our results differ from the results previously reported. This could be due to the genetic instability of iPS cells.

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