gms | German Medical Science

Background: Phototherapy is a well-recognized treatment option. Endonasal phototherapy has been introduced to treat immune-mediated mucosal diseases, such as allergic rhinitis. High energetic radiations due to phototherapy may cause DNA and protein damages. Evaluating damage and repair process in human tissues after phototherapy is an important safety aspect. In this study, we investigated the protein profile of untreated and treated epithelial cells.

Methods: Human airway epithelial cells were cultivated and treated with a combination of UV-A (25%), UV-B (5%) and visible light (70%) for different time periods. Proteins from these samples were extracted and separated by immobilized pH gradient-based two-dimensional difference gel electrophoresis (2-D DIGE). Resulting 2D-gel images were statistically analyzed using Delta2D software and differently expressed protein spots were analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/TOFMS) and identified.

Results: A reference map of approximately 2000 proteins could be established. Several proteins were significantly changed (2-fold, P <0.05) in UV-treated epithelial cells compared to untreated cells. These proteins addressed for identification by MALDITOF/TOF-MS.

Conclusion: Major significantly changed proteins and their biological functions will be discussed regarding the effect of different energetic radiations. The role of these proteins as therapeutic or toxicity markers have been analyzed in the study. New treatment regimens similar in duration used for the treatment of epithelial cells may be useful for immune-mediated or inflammatory diseases of the airways, such as nasal polyps or chronic rhinosinusitis or allergic rhinitis.

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