gms | German Medical Science

86. Jahresversammlung der Deutschen Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie e. V.

Deutsche Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie e. V.

13.05. - 16.05.2015, Berlin

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Interaction of H2O2 and TGF-beta1 in an in-vitro wound model of respiratory epithelial cells

Meeting Abstract

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  • corresponding author Yucheng Yang - Department of Otorhinolaryngology,The First Affiliated Hospital, Chongqing Medic, Chongqing, China
  • Achim G. Beule - Dept. ENT, Head & Neck Surgery, University Hospital Greifswald, Greifswald
  • Werner Hosemann - Dept. ENT, Head & Neck Surgery, University Hospital Greifswald, Greifswald
  • Katrin Darm - ENT Research Laboratory, University Hospital Greifswald, Greifswald
  • Guohua Hu - Department of Otorhinolaryngology,The First Affiliated Hospital, Chongqing Medic, Chongqing, China
  • Suling Hong - Department of Otorhinolaryngology,The First Affiliated Hospital, Chongqing Medic, Chongqing, China
  • Christian Scharf - ENT Research Laboratory, University Hospital Greifswald, Greifswald

Deutsche Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie. 86. Jahresversammlung der Deutschen Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie. Berlin, 13.-16.05.2015. Düsseldorf: German Medical Science GMS Publishing House; 2015. Doc15hnod083

doi: 10.3205/15hnod083, urn:nbn:de:0183-15hnod0833

Veröffentlicht: 26. März 2015

© 2015 Yang et al.
Dieser Artikel ist ein Open Access-Artikel und steht unter den Creative Commons Lizenzbedingungen. Er darf vervielfältigt, verbreitet und öffentlich zugänglich gemacht werden, vorausgesetzt dass Autor und Quelle genannt werden. Lizenz-Angaben siehe http://creativecommons.org/licenses/by-nc-nd/3.0/.

Gliederung

Text

Introduction: Human airway epithelium acts as a protective barrier and is subject to injury during exposure to a variety of inhaled toxins or infectious agents, such as reactive oxygen species (ROS). Repair of epithelial injury requires a wound repair process, in which TGF-beta1 exerts an important function. However stimulating efficacy and the interaction between ROS and TGF-beta1 in airway wound healing remain not clearly described.

Methods: Two kinds of airway epithelial cells, S9 and A549, were treated with hydrogen peroxide (H2O2) and /or TGF-beta1 and with/without corresponding inhibitors (catalase, SB431542). An in vitro wound model was used to figure out the effect on wound healing; the number of living cells was measured by CASY; the morphology of cells was analyzed by microscopy; intracellular ROS changes were identified by 2-Cys Prx Redoxblot; the TGF-beta1 level in supernatants was tested by ELISA.

Results: Cell growth was inhibited by H2O2 in a time and dose-dependent manner in both S9 and A549 cell lines. TGF-beta1 induced S9 epithelial cell growth during the first 24 hours, but inhibited cell growth after 72 hours. In contrast A549 cells growth was time-dependently inhibited. TGF-beta1 also induced morphologic changes of S9 cells into a spindly shape. Both H2O2 and TGF-beta1 affected the wound healing capacity significantly and TGF-beta1 expression and level of ROS were affected in S9 and A549 epithelial cell lines.

Conclusions: ROS and TGF-beta1 show cellular interactions in airway epithelial cell growth and wound healing.

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