Artikel
Characterization of HPV-positive head and neck squamous cell carcinoma cell lines
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Veröffentlicht: | 22. April 2010 |
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Gliederung
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Introduction: Antiviral therapy has a possible beneficial therapeutic effect in patients with human papillomavirus (HPV)-positive head and neck squamous cell carcinomas (HNSCC). Because the cellular features responsible for this effect are not sufficiently understood, it is essential to investigate HPV-positive and -negative tumor cell lines. To what extent the genomic and phenotypic features of these cell lines correspond to those of primary tumors is unknown and has been investigated in this study.
Methods: Three HPV-16-positive and 4 HPV-negative HNSCC cell lines were analyzed, and the obtained results were compared with data previously gathered on primary tumor samples. HPV type and physical status were determined using fluorescence in situ hybridisation (FISH), PCR and amplification of papillomavirus oncogenic transcripts (APOT) analysis. Chromosome 1 and 7 copy numbers were determined using FISH. Expression of proteins in the pRb- and p53-pathways and cytokeratines were determined using immunocytochemistry.
Results: Our experiments revealed that 1) all HPV-positive cell lines have punctate FISH patterns indicating integrated oncogenic HPV, and that the integration status was confirmed in 2 cell lines by APOT, 2) the HPV-positive cell lines exhibit aneusomy for chromosomes 1 and 7, 3) HPV influences expression of proteins in the pRb- and p53-pathways in the cell lines in a similar way as in primary tumors and 4) cytokeratine immunostainings indicate a squamous epithelial origin of the cell lines.
Conclusions: The investigated HPV-positive HNSCC cell lines are suitable for research on the therapeutical effects of antiviral therapy.