Artikel
IL-6 production from monocytes is correlated with worsened survival in Head and Neck Squamous Cell Carcinomas
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Veröffentlicht: | 24. April 2007 |
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Gliederung
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Introduction: Head and Neck Squamous Cell Carcinomas (HNSCC) are infiltrated with cells from the Mononuclear phagocyte system (MNP) which have the ability to bind and, if activated, kill tumor cells. IL-6 and MCP-1 are cytokines which play a role in the development and metastasis of cancer.
Methods: An organ culture method was used by which small parts of tissues, either from Head and Neck Squamous Carcinoma (HNSCC) or benign mucosa, could be maintained viable in vitro as Fragment (F)-spheroids. F-spheroids stimulate autologous monocytes to secrete interleukin (IL)-6 and monocyte chemotactic protein (MCP)-1 in vitro. We analysed the IL-6 and MCP-1 production from monocytes in co-culture with autologous F-spheroids in a cohort of patients from 1998-2005 all treated for HNSCC.
Results: We found that an increased production of IL-6 by autologous monocytes, in co-culture with F-spheroids is correlated with recurrence of disease(p<0,05).
Conclusion: Monocytes in co-culture with autologous F-spheroids produce IL-6 and MCP-1. The IL-6 production is shown to be a prognostic factor in vitro in HNSCC.
Unterstützt durch: University in Bergen, Norway