gms | German Medical Science

87. Jahresversammlung der Deutschen Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie e. V.

Deutsche Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie e. V.

04.05. - 07.05.2016, Düsseldorf

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Three-Dimensional Culture of Human Skin Fibroblasts on Bio-Resorbable Electrospun PLGA-Tissues in Experimental Otology

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  • corresponding author presenting/speaker Justus Ilgner - University Hospital Aachen RWTH, Department of Otorhinolaryngology and Plastic Surgery for the Head and Neck, Aachen, Germany
  • author Anmin Shen - University Hospital Aachen RWTH, Department of Otorhinolaryngology and Plastic Surgery for the Head and Neck, Aachen, Germany
  • author Magnus Kruse - Institute for Textile Technology, RWTH Aachen, Aachen, Germany
  • author Annette Kolkmann - ITA GmbH, Aachen, Germany
  • author Frank Portheine - Surgitaix AG, Herzogenrath, Germany
  • author Max Kinzius - Surgitaix AG, Herzogenrath, Germany
  • author Bernd Schnieber - Spiggle und Theis GmbH, Overath, Germany
  • author Martin Westhofen - University Hospital Aachen RWTH, Department of Otorhinolaryngology and Plastic Surgery for the Head and Neck, Aachen, Germany

German Society of Oto-Rhino-Laryngology, Head and Neck Surgery. 87th Annual Meeting of the German Society of Oto-Rhino-Laryngology, Head and Neck Surgery. Düsseldorf, 04.-07.05.2016. Düsseldorf: German Medical Science GMS Publishing House; 2016. Doc16hno20

doi: 10.3205/16hno20, urn:nbn:de:0183-16hno202

Veröffentlicht: 7. September 2016

© 2016 Ilgner et al.
Dieser Artikel ist ein Open-Access-Artikel und steht unter den Lizenzbedingungen der Creative Commons Attribution 4.0 License (Namensnennung). Lizenz-Angaben siehe http://creativecommons.org/licenses/by/4.0/.

Gliederung

Text

Introduction: The use of Titanium ossicular replacement prostheses is a reliable method to restore sound transmission in reconstructive middle ear surgery. In some cases, however, insufficient adhesion of titanium surfaces to neighbouring biological structures results in implant failure. Bio-resorbable electrospun textiles provide a potential solution to this problem; thanks to their bonding properties. The objective of this study is to compare the growth of human skin fibroblasts on three-dimensional PLGA tissues versus two-dimensional titanium platelets.

Methods: Polylactide-co-Glycolide (PLGA) tissues were generated by electrospinning and cultured with human skin fibroblasts (HDF). Cultured cells were assessed by cell count, their vitality evaluated in Prestoblue assay and cell morphology on PLGA tissues measured by two-photon fluorescence microscopy as well as scanning electron microscopy (SEM). Vitality of HDF and their affinity to 3D-PLGA tissues were compared to 2D-titanium platelets.

Results: HDF display a significantly greater affinity to PLGA tissues compared to titanium platelets on day 1. Furthermore, they display a significantly higher proportion of vital cells on PLGA compared to titanium by day 7. HDF can penetrate in between tissue fibres and therefore grow in three-dimensional patterns.

Conclusion: The introduction of bio-resorbable electrospun polymer fibres provides a promising modality to promote integration of ossicular chain prosthesis in surrounding middle ear tissue.