Artikel
Biomarker to detect genotoxic activities in the oral cavity in correlation to the oral hygiene
Suche in Medline nach
Autoren
Veröffentlicht: | 22. September 2005 |
---|
Gliederung
Text
Background: Valid biomarker for recognition of high risk patients can become in future the basis of prevention-studies. Until now the influence of oral health including oral hygiene, dental status and human oral microflora on biomarker in the oral cavity is unknown. Hence, we evaluated two different biomarkers (micronucleus – assay and Ames – test for saliva testing), which are routinely used in our department to detect humans with an increased cancer risk in the upper aerodigestive tract to examine the influence of oral hygiene on the carcinogenesis in the oral cavity.
Patients and Methods: 100 healthy probands with different oral hygiene and dental status were included in the above mentioned study. The micronucleus frequency was counted up to thousand buccal cells in the exfoliative cytology. Saliva specimen were analyzed for genotoxic activity using the Ames – test in different settings (Salmonella typhimurium TA 98 and TA 100 with or without S9 – mixture) The measured values were correlated to the oral health using the SPSS software pack.
Results: Study participants with a bad oral hygiene below the average of the normal population including dental caries and periodontal status showed significantly increased genotoxic activity of saliva specimen and an increased rate of buccal micronuclei as a sign of chromosomal damage of the mucosa. Participants with composite fillings of decayed teeth showed also increased measurements of the above mentioned biomarker in correlation to patients with amalgam filled teeth.