Artikel
The effect of VEGF-C on secretion of VEGF-A of the RPE
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Autoren
Veröffentlicht: | 22. September 2004 |
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Gliederung
Text
Objective
The Effect of VEGF-A on endothelial cells in the development of CNV is well described. RPE-cells are a main source for VEGF-A and express the receptors VEGFR-1 and VEGFR-2 as well. It has been reported that cells from excised CNV-Membranes show a higher expression of VEGFR-2. Purpose of this study is the analysis of possible autocrine "feed-back-loops" on the VEGF production by an increased secretion of VEGF from RPE-cells. These feed-back-loops may act as inducers of CNV.
Methods
VEGF-secretion was measured by the increase of concentration of VEGF in culture medium from 105 ARPE 19 cells. Concentration of VEGF was measured using a Sandwich ELISA.
Results
After the last exchange of culture medium the concentration of VEGF increased continuously and reached a maximum of 295pg/ml after 8 hours. By adding the VEGF analogon VEGF-C this basal secretion could be elevated up to 127,5% of control. After 8 hours under these conditions the VEGF concentration reached 375pg/ml.
Conclusions
VEGF-C which has a high affinity to VEGF-R2 stimulates the secretion of VEGF-A in RPE-cells efficiently. Thus, the VEGF-C dependend control of the VEGF-A secretion of the RPE is another possible mechanism causing induction of CNV in AMD.