gms | German Medical Science

102. Jahrestagung der DOG

Deutsche Ophthalmologische Gesellschaft e. V.

23. bis 26.09.2004, Berlin

Expression of p63 in conjunctival intraepithelial neoplasia and squamous carcinoma

Meeting Abstract

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  • corresponding author C. Auw-Hädrich - Universitäts-Augenklinik Freiburg
  • R. Sundmacher - Universitäts-Augenklinik Düsseldorf
  • T. Reinhard - Universitäts-Augenklinik Freiburg

Evidenzbasierte Medizin - Anspruch und Wirklichkeit. 102. Jahrestagung der Deutschen Ophthalmologischen Gesellschaft. Berlin, 23.-26.09.2004. Düsseldorf, Köln: German Medical Science; 2004. Doc04dogP 011

Die elektronische Version dieses Artikels ist vollständig und ist verfügbar unter: http://www.egms.de/de/meetings/dog2004/04dog502.shtml

Veröffentlicht: 22. September 2004

© 2004 Auw-Hädrich et al.
Dieser Artikel ist ein Open Access-Artikel und steht unter den Creative Commons Lizenzbedingungen (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.de). Er darf vervielfältigt, verbreitet und öffentlich zugänglich gemacht werden, vorausgesetzt dass Autor und Quelle genannt werden.


Gliederung

Text

Objective

P63is a homologue of the tumor suppressor gene p53 which is expressed in human basal squamous epithelium. P63 is suggested to play a role in the development of squamous epithelium and despite the homology to p53 rather that of an oncogene. This study investigated the expression of p63 in conjunctival intraepithelial neoplasia of different grades and conjunctival squamous carcinoma and its correlation to the proliferation marker MIB1.

Methods

15 conjunctival specimen which were excised with the suspicion of conjunctival intraepithelial neoplasia (CIN) and squamous carcinoma, respectively, were diagnosed histologically as follows: 2 squamous carcinomas of the conjunctiva, 1 CIN grade I, 3 CIN grade II, 7 CIN grade III, 1 CIN with beginning invasion and 1 normal conjunctiva without any dysplasia. These formalin-fixed specimen and 4 macroscopically normal postmortem conjunctival specimen were stained immunohistochemically with antibodies against p63 and MIB1.

Results

Most of the hyperplastic cells of the CIN specimen were stained positively with antibodies against p63, especially in the lower two-third. The lower the grade of differentiation, the more the upper cell layers were positively stained. All layers of the invasive areas of the two squampus cell carcinomas stained strongly positive, whereas the non-invasive areas on the same slide showed less staining. Normal conjunctiva adjacent to the dysplastic areas showed even less staining intensitivity confined to the basal cell layer, which was also the case in the macroscopically normal postmortem conjunctival specimens. Compared to the p63 staining clearly less MIB1-positivity was seen in the basal and sometimes also in the middle layer of the neoplastic specimen as well as in the postmortem controls.

Conclusions

In conjunctival intraepithelial neoplasia and squamous carcinoma of the conjunctiva p63 is preferentially expressed in the neoplastic immature cells of squamous lineage and seems not to be linked to cell proliferation.