gms | German Medical Science

Deutscher Kongress für Orthopädie und Unfallchirurgie (DKOU 2022)

25. - 28.10.2022, Berlin

Artikel

Artikel empfehlen

Porcine Mandibular BMSC-Derived EVs Can Promote the Proliferation and Osteogenic Differentiation Capacity of Porcine Tibial-Derived BMSC

Meeting Abstract

Suche in Medline nach

  • presenting/speaker Qun Zhao - Klinik für Unfall- und Wiederherstellungschirurgie, Uniklinik RWTH Aachen, Aachen, Germany
  • Xing Zhang - Klinik für Unfall- und Wiederherstellungschirurgie, Uniklinik RWTH Aachen, Aachen, Germany
  • Nan Zhou - Klinik für Unfall- und Wiederherstellungschirurgie, Uniklinik RWTH Aachen, Aachen, Germany
  • Frank Hildebrand - Klinik für Orthopädie, Unfall- & Wiederherstellungschirurgie, Uniklinik RWTH Aachen, Aachen, Germany
  • Johannes Greven - Klinik für Orthopädie, Unfall- & Wiederherstellungschirurgie, Uniklinik RWTH Aachen, Aachen, Germany

Deutscher Kongress für Orthopädie und Unfallchirurgie (DKOU 2022). Berlin, 25.-28.10.2022. Düsseldorf: German Medical Science GMS Publishing House; 2022. DocAB56-742

doi: 10.3205/22dkou423, urn:nbn:de:0183-22dkou4233

Veröffentlicht: 25. Oktober 2022

© 2022 Zhao et al.
Dieser Artikel ist ein Open-Access-Artikel und steht unter den Lizenzbedingungen der Creative Commons Attribution 4.0 License (Namensnennung). Lizenz-Angaben siehe http://creativecommons.org/licenses/by/4.0/.

Gliederung

Text

Objectives: Existing research suggests that bone marrow-derived mesenchymal stem cells (BMSC) may promote endogenous bone repair through secretion of factors that stimulate endogenous repair processes or directly stimulate new bone formation through differentiation into osteoblast-like cells. However, the osteogenic potential of BMSC varies among different tissue sources. The main aim of this study is to investigate the difference in proliferation and osteogenic differentiation capacity between porcine mandibular-derived BMSC and tibial-derived BMSC.

Methods: Bioinformatics analysis of the GSE81430 dataset from the Gene Expression Omnibus (GEO) database was performed. BMSC cells were isolated from bone marrow samples from porcine mandibles and tibias (registered at the State Office for Nature, Environment and Consumer Protection North Rhine-Westphalia (LANUV) 81-02.04.2020.A215). Cell morphology, proliferation, and osteogenic differentiation were evaluated in both groups. Expressions of related mRNAs and proteins were detected by qPCR and WB respectively. Cell morphology, proliferation, and osteogenic differentiation capacity were also observed in tibial BMSC with mandibular BMSC derived EVs added.

Results: Bioinformatics analysis revealed that TLR4 and NF-kB pathway showed great significant differences. The mandibular BMSC group showed stronger proliferation and osteogenic differentiation capacity compared to the tibial BMSC group. The qPCR and WB revealed a significant decrease in the expressions of the TLR4 and NF-kB in the mandibular BMSC group. Performed ELISAs showed a significant decrease in the expressions of the NF-kB in the mandibular BMSC group. The proliferation and osteogenic differentiation capacity were enhanced, and qPCR and WB revealed a significant decrease in the expressions of the TLR4 and NF-kB in the tibial BMSC group with mandibular BMSC derived EVs added.

Conclusion: Our results indicated that mandibular BMSC derived EVs can promote proliferation and osteogenic differentiation of tibial BMSC in vitro. This result provides an implication for us to understand the mechanism of the decreased osteogenic ability via TLR4/NF-kB signaling pathway activation and may provide a new thought for bone defect or fracture treatment.