Artikel
The influence of mesenchymal stem cells on tendon-to-bone-healing by attaching bursal tissue to rotator cuff tears in an animal model
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Autoren
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Anja Zhang
- Klinikum der Universität München, Klinik für Orthopädie, München, Germany
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Andreas Ficklscherer
- Klinikum der Universität München, Klinik für Orthopädie, München, Germany
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Thomas Beer
- Klinikum der Universität München, Klinik für Orthopädie, München, Germany
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Mehmet Gülecyüz
- Klinik für Orthopädie, München, Germany
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Matthias Pietschmann
- Klinikum der Universität München, Klinik für Orthopädie, München, Germany
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Volkmar Jansson
- Klinikum der Universität München, Klinik für Orthopädie, München, Germany
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Peter E. Müller
- Klinikum der Universität München, Klinik für Orthopädie, München, Germany
| Veröffentlicht: | 6. November 2018 |
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Gliederung
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Objectives: The aim of this study is to analyse the influence of migration of mesenchymal stem cells from autologous bursal tissue on tendon to bone healing after rotator cuff repair in a rat model. Another aspect of the study investigates the difference in using inflammation bursal tissue or bland bursal tissue for application onto the tendon-bone interface.
Methods: 204 Sprague-Dawley rats were randomly assigned to either untreated, bland bursal tissue application or inflamed bursal tissue application group in a rotator cuff repair. After separating the supraspinatus tendon (SSP) close to the greater tubercle, the tendon was re-attached either unaltered, with a bland bursal tissue patch or an inflammation-induced bursal patch sewn onto the interface. Histologic Analysis 1 week and 7 weeks after SSP reinsertion and biomechanical testing of the tendon 6 and 7 weeks after reinsertion was performed.
Results and conclusion: Histologic results show a significant larger mean interface region, represented by collagen II in the tissue, in the bland bursal and inflammation group after 1 week in comparison to the control groups (p< 0.05). Similar results could be found in bland bursal and inflammation bursal tissue after 7 weeks (p< 0.05). No significant difference was detected between inflammation bursal tissue and bland bursal tissue groups after 1 week and 7 weeks.
Tendon stiffness differed significantly (p< 0.05) between bland bursal and control group as well as between inflammation group and control group (p< 0.05). Tendon viscoelasticity (QLV) was significantly higher in the bland bursa groups with more QLV after 6 weeks rather than after 7 weeks.
The study results show migration of bursal tissue cells to tendon-bone interface after rotator cuff repair with better tendon-to bone healing attaching bland bursal tissue. Stem cell migration from bland bursal tissue might aid in faster healing processes of tendon-cartilage-bone interfaces in rat models and may be an indication for applying bursal tissue or bursal cells by injection in clinical use.
