gms | German Medical Science

Deutscher Kongress für Orthopädie und Unfallchirurgie (DKOU 2018)

23.10. - 26.10.2018, Berlin

The bone marrow microenvironment in patients undergoing hip endoprosthesis surgery due to coxarthrosis or femoral neck necrosis

Meeting Abstract

  • presenting/speaker Marietta Herrmann - IZKF Group Tissue Regeneration in Musculoskeletal Diseases, Orthopedic Center for Musculoskeletal Research, Universität Würzburg, Würzburg, Germany
  • Boris M. Holzapfel - Orthopädische Klinik König Ludwig Haus, Universität Würzburg, Würzburg, Germany
  • Theresa Kreuzahler - IZKF Group Tissue Regeneration in Musculoskeletal Diseases, Orthopedic Center for Musculoskeletal Research, Universität Würzburg, Würzburg, Germany
  • Maximilian Rudert - Orthopädische Klinik König-Ludwig-Haus, Orthopädisches Zentrum für Muskuloskelettale Forschung, Lehrstuhl für Orthopädie der Universität Würzburg, Würzburg, Germany
  • Drenka Trivanovic - IZKF Group Tissue Regeneration in Musculoskeletal Diseases, Orthopedic Center for Musculoskeletal Research, Universität Würzburg, Würzburg, Germany

Deutscher Kongress für Orthopädie und Unfallchirurgie (DKOU 2018). Berlin, 23.-26.10.2018. Düsseldorf: German Medical Science GMS Publishing House; 2018. DocPT12-949

doi: 10.3205/18dkou592, urn:nbn:de:0183-18dkou5922

Veröffentlicht: 6. November 2018

© 2018 Herrmann et al.
Dieser Artikel ist ein Open-Access-Artikel und steht unter den Lizenzbedingungen der Creative Commons Attribution 4.0 License (Namensnennung). Lizenz-Angaben siehe http://creativecommons.org/licenses/by/4.0/.


Gliederung

Text

Objectives: Sharing anatomical location in bone marrow (BM), hematopoietic and mesenchymal progenitor cells are critically involved in bone regeneration and degenerative disease progression. Here, we were interested how mesenchymal and hematopoietic progenitors are influenced by the diseases state of patients undergoing hip endoprosthesis surgery.

Methods: BM reaming was collected from patients after informed consent (n=13). Disease state of patients was evaluated by scoring of x-rays according the Kellgren and Lawrence system for classification of osteoarthritis. Mononuclear cells (MNCs) were isolated by density centrifugation. Frequency of mesenchymal stromal cells (MSCs) was determined by fibroblastic colony forming units (CFU-F, n=12) and flow cytometry (n=4). Different marker combinations were tested for the identification of MSCs within MNCs, including CD13, CD146, CD44, CD73 and CD271 as putative MSC marker in absence of hematopoietic markers (CD45, CD34). Hematopoietic cell populations were investigated by short-term colony forming cell (CFC) assay in methylcellulose-based medium (n=5) and flow cytometry analysis of CD45 and CD34 expression (n=4). Data was tested for normal distribution and statistical significance analyzed by unpaired t-test. All values are given as mean ± standard deviation.

Results and conclusion: MSCs could be expanded from all donors with an average CFU-F efficiency of 0.021±0.007% of total MNCs. We found that CFU-F efficiency was correlated with diseases state of patients; CFU-F efficiency was lower (0.020±0.008%) in patients with grade 4 arthrosis in comparison to patients with grade 3 arthrosis (0.024±0.008%, p>0.05). Likewise, a trend of reduced CFU-F efficiency was found in osteoporotic donors (0.016±0.007%) compared to the non-osteoporosis group (0.023±0.007%; p>0.05). Testing different combinations of putative MSC marker revealed that the CD45-CD13+CD271+ population (0.027±0.029%) was most closely correlated with the CFU-F efficiency, which will be confirmed by FACS sorting in future experiments. Within total MNCs, frequency of hematopoietic progenitors (CD45+CD34+), was 0.303±0.202%, while 0.246±0.048% were capable to form hematopoietic colonies. Although myeloid CFU granulocyte/erythrocyte/macrophage/megakaryocyte (CFU-GEMM) were most frequent (0.18±0.056%), replating assays revealed that only hematopoietic cells committed toward macrophage lineage retain their growth ex vivo.

Our data suggest that mesenchymal and hematopoietic progenitors can be influenced by the inflammation-associated BM microenvironment caused by degenerative events in the femoral neck. Future studies will further investigate how specific cell populations correlate with disease progression and how these cells might eventually be therapeutically targeted.