gms | German Medical Science

Deutscher Kongress für Orthopädie und Unfallchirurgie (DKOU 2018)

23.10. - 26.10.2018, Berlin

Meniscus 3D model by wedge-shape compression of a MSC-laden collagen type I gel

Meeting Abstract

  • presenting/speaker Antje Kremer - Lehrstuhl für Tissue Engineering und Regenerative Medizin, Würzburg, Germany
  • Jenny Reboredo - Lehrstuhl für Tissue Engineering und Regenerative Medizin, Würzburg, Germany
  • Jan Hansmann - Translationszentrum Regenerative Therapien, Fraunhofer-Institut für Silicatforschung ISC, Würzburg, Germany
  • Marc Möllmann - Translationszentrum Regenerative Therapien, Fraunhofer-Institut für Silicatforschung ISC, Würzburg, Germany
  • Marietta Herrmann - IZKF Gruppe Tissue Regeneration in Musculoskeletal Diseases, Orthopädisches Zentrum für Muskuloskelettale Forschung, Würzburg, Germany
  • Heike Walles - Lehrstuhl für Tissue Engineering und Regenerative Medizin, Translationszentrum Regenerative Therapien, Fraunhofer-Institut für Silicatforschung ISC, Würzburg, Germany

Deutscher Kongress für Orthopädie und Unfallchirurgie (DKOU 2018). Berlin, 23.-26.10.2018. Düsseldorf: German Medical Science GMS Publishing House; 2018. DocGF16-523

doi: 10.3205/18dkou488, urn:nbn:de:0183-18dkou4881

Veröffentlicht: 6. November 2018

© 2018 Kremer et al.
Dieser Artikel ist ein Open-Access-Artikel und steht unter den Lizenzbedingungen der Creative Commons Attribution 4.0 License (Namensnennung). Lizenz-Angaben siehe http://creativecommons.org/licenses/by/4.0/.


Gliederung

Text

Objectives: Meniscal injuries often result in the deterioration of meniscal tissue and consequently of articular cartilage. The healing potential of the avascular region of the meniscus is limited and no optimal treatment strategy is available. Replacing torn menisci by a tissue-engineered 3D meniscus implant seems to be a promising strategy.

A 3D meniscus model needs an appropriate biomaterial for 3D cell culture. The organic material of meniscus mainly consists of collagen, especially collagen type I (Col I). The aim of this study was to build up a 3D meniscus model by wedge-shape compression of a cell-laden Col I gel. To improve the mechanical properties of gels, dynamic culture by biomechanical stimulation was tested.

Methods: As meniscal cells are a limited cell source and have limited proliferation potential, mesenchymal stem cells (MSC) - providing fibrochondrogenic potential were isolated from human bone marrow after informed consent of the patient, expanded in passage 1 and their chondrogenic potential was assessed. Human MSC were embedded in Col I gel and MSC-laden Col I gels were compressed to a wedge-shaped 3D meniscus model.

Wedge-shaped MSC-laden Col I gel were cultured static or dynamic by biomechanical stimulation. During three weeks of 3D culture, the shrinkage behavior of MSC-laden Col I gels was analyzed. On day 21, MSC-laden Col I gels were investigated for cell viability, expression of meniscal markers by immunohistochemical stainings and GAG/DNA content.

Results and conclusion: MSC were successfully isolated from human bone marrow and their chondrogenic potential was confirmed by Alcian Blue staining of sGAG and expression of Col II.

Wedge-shape compression in a customized bioreactor system with a movable wedge was successfully established. MSC-laden Col I gels were compressed to a wedge-shaped 3D meniscus model. During three weeks of static 3D culture, the supplementation of transforming growth factor beta-3 (TGF beta-3) led to increased shrinkage of the wedge-shaped MSC-laden Col I gel (37.3±0.8%). On day 21, wedge-shaped MSC-laden Col I gels showed high viability. The fibrochondrogenic properties were confirmed by immunohistochemical detection of meniscal markers (Col I, Col II and Aggrecan) and significant GAG increase.

The here reported study suggested a wedge-shaped MSC-laden Col I gel as suitable biomaterial for meniscus tissue engineering. We are currently investigating whether mechanical stimulation can further improve the model and promote tissue remodeling in the direction of meniscal tissue.

As no optimal treatment strategy of meniscal injuries is available so far, this regenerative approach for replacing torn menisci by a 3D meniscus model based on a wedge-shaped cell-laden Col I gel is a promising strategy.