Artikel
Discovery of glycan biomarkers in serum of osteonecrosis of the femoral head
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Veröffentlicht: | 23. Oktober 2017 |
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Gliederung
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Objectives: We aimed to examine the serum glycosylation alteration in the development of steroid-induced 0steonecrosis of the femoral head (SONFH) and identify N-glycan as the potential biomarkers in diagnosing SONFH.
Methods: A training set containing 27 serum samples from steroid induced ONFH patients and 25 from gender and age matched controls were collected and used. Glycomic and site-specific glycosylation of immunoglobulins are examined for potential markers. LC-MS is used for the global glycomic profiling of the serum. LC-MS/MS using multiple reaction monitoring (MRM) is used for quantitating site-specific glycosylation of serum immunoglobulins.
Results: Results: The glycomic analysis yielded 14 N-glycan compositions and MRM yielded 8 glycopeptides that were altered between cases and controls with statistical significance. The increase of the non-sialylated, non-fucosylated N-glycans and decrease of the fucosylated Nglycans are associated with the development of ONFH. The best N-glycan marker candidate is N5410 (P<0.0001, AUC=0.85). An additional nine N-glycan and three glycopeptides were found to be promising candidates.
Conclusion: Glycosylation is a post-translational protein modification that appears to be affected by ONFH. The results are consistent with recent reports that specific glycosylation profiles are related to various pathological states. Further studies with a testing set will provide validation of these potential markers.