Artikel
Distribution of fibroblasts and fibrocytes in the marginal synovium in rats during the postnatal period after antenatal antigenic stimulation
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Autoren
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Andrii Fedotchenko
- Zeisigwaldkliniken Bethanien Chemnitz, Rheumatology, Chemnitz; Zaporizhzhia State Medical University, Zaporizhzhia
| Veröffentlicht: | 31. August 2022 |
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Gliederung
Text
Introduction: Fibroblasts and fibrocytes play an important role in joint tissue development, differentiation, remodeling, and repair. We studied joint capsule morphology using hip joints of Wistar rats during the postnatal period in norm and after antigenic stimulation, which was modeled through the transuterine intrafetal interscapular subcutaneous injection of antigen (0.05 ml of human normal immunoglobulin) for the rat fetus on the 18th day of its antenatal life under general anesthesia and sterile conditions via laparotomy for the pregnant female (Voloshynʼs method (1981). The control group of rats was injected with 0.05 ml of physiological saline in the same manner.
Methods: The joints were fixed, decalcified and dehydrated. Paraffin-embedded tissue sections were processed with hematoxylin-eosin stain. Cells were analyzed by light microscopy (x100) using the morphometrics and statistical processing. The parameters of intact and control rats did not differ significantly from each other.
Results: The maximum number of young fibroblasts in intact rats was in newborns, in antigen-suppressed – on the seventh day. The number of young fibroblasts in antigen-suppressed rats till the 45th day was less compared to intact rats. The number of mature fibroblasts in antigen-suppressed rats on the first, seventh and 30th day was more, but on the 14th day – less compared to intact rats. The number of fibrocytes in antigen-suppressed rats on the first and 30th day was more, on the seventh day – less compared to intact rats. The recovery of the number of mature fibroblasts and fibrocytes in antigen-suppressed rats in relation to the intact rats was observed on the 45th day, young fibroblasts – on the 60th day.
Conclusion: Changes in cellular distribution after antigenic stimulation might indicate the immunobiological tensions in the marginal synovium, due to the possible influence of the antigen-activated fibroblasts and fibrocytes on other cells and extracellular matrix. It could affect the structure of the marginal transitional zone and pose a threat of excessive invasion of the antigen-suppressed joint capsule into the articular cartilage.
Disclosures: No conflict of interest.
