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Deutscher Rheumatologiekongress 2021, 49. Kongress der Deutschen Gesellschaft für Rheumatologie (DGRh), 35. Jahrestagung der Deutschen Gesellschaft für Orthopädische Rheumatologie (DGORh), Wissenschaftliche Herbsttagung der Gesellschaft für Kinder- und Jugendrheumatologie (GKJR)

15.09. - 18.09.2021, virtuell

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Phenotypic analysis of circulating plasma cells after immunization with anti-SARS-CoV-2 mRNA vaccines in patients with chronic inflammatory diseases

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  • Maria S. Ciripoi - UKSH, Medical Department I, Department for Rheumatology and Clinical Immunology, Kiel
  • Ulf M. Geisen - UKSH, Medical Department I, Department for Rheumatology and Clinical Immunology, Kiel
  • Lena Vullriede - UKSH, Medical Department I, Department for Rheumatology and Clinical Immunology, Kiel
  • Hayley M. Reid - UKSH, Medical Department I, Department for Rheumatology and Clinical Immunology, Kiel
  • Dennis K. Berner - UKSH, Medical Department I, Department for Rheumatology and Clinical Immunology, Kiel
  • Florian Tran - UKSH, Department for Internal Medicine I, Kiel; Christian-Albrechts-Universität, Institute for Clinical Molecular Biology, Kiel
  • Melike Sümbül - UKSH, Department for Dermatology, Kiel
  • Annika Schaffarzyk - UKSH, Department for Rheumatology and Clinical Immunology, Kiel
  • Jan H. Schirmer - UKSH, Medical Department I, Department for Rheumatology and Clinical Immunology, Kiel
  • Rainald Zeuner - UKSH, Medical Department I, Department for Rheumatology and Clinical Immunology, Kiel
  • Anette Friedrichs - UKSH, Department for Internal Medicine I, Kiel
  • Andrea Steinbach - UKSH, Medical Department I, Department for Rheumatology and Clinical Immunology, Kiel
  • Sascha Gerdes - UKSH, Department for Dermatology, Kiel
  • Petra Bacher - UKSH, Institute of Immunology, Kiel
  • Stefan Schreiber - UKSH, Department for Internal Medicine I, Kiel; Christian-Albrechts-Universität, Institute for Clinical Molecular Biology, Kiel
  • Bimba F. Hoyer - UKSH, Medical Department I, Department for Rheumatology and Clinical Immunology, Kiel

Deutsche Gesellschaft für Rheumatologie. Deutsche Gesellschaft für Orthopädische Rheumatologie. Gesellschaft für Kinder- und Jugendrheumatologie. Deutscher Rheumatologiekongress 2021, 49. Kongress der Deutschen Gesellschaft für Rheumatologie (DGRh), 35. Jahrestagung der Deutschen Gesellschaft für Orthopädische Rheumatologie (DGORh), Wissenschaftliche Herbsttagung der Gesellschaft für Kinder- und Jugendrheumatologie (GKJR). sine loco [digital], 15.-18.09.2021. Düsseldorf: German Medical Science GMS Publishing House; 2021. DocCO.08

doi: 10.3205/21dgrh008, urn:nbn:de:0183-21dgrh0085

Veröffentlicht: 14. September 2021

© 2021 Ciripoi et al.
Dieser Artikel ist ein Open-Access-Artikel und steht unter den Lizenzbedingungen der Creative Commons Attribution 4.0 License (Namensnennung). Lizenz-Angaben siehe http://creativecommons.org/licenses/by/4.0/.

Gliederung

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Introduction: Plasma cells and plasmablasts (PCs) in the peripheral blood have been shown to exhibit a phenotype different to homoeostatic PC populations of the blood in response to vaccinations, i.e. after tetanus vaccinations. These cells present with a phenotype of newly activated plasmablasts and in parallel with phenotypic features of mature PCs found in the bone marrow. It is unclear whether vaccination with the new mRNA vaccines will lead to the same immunological phenomena and whether these might be changed in the context of chronic inflammatory diseases (CID) and anti-cytokine therapies. The aim of this study is to analyze the phenotype of SARS-CoV-2 specific PCs in the peripheral blood of patients and healthy individuals after mRNA vaccinations.

Methods: 24 controls and 22 patients with CIDs were recruited. All subjects were immunized by official national vaccination centers with mRNA vaccines. Peripheral blood samples were taken seven days after the second vaccination. Peripheral Blood Mononuclear Cells (PBMCs) wned with SARS-CoV-2 spike-S1 protein as well as for the presence of CD3, CD14, CD19, CD20, CD27, CD95, CD138, HLA-DR, IgD and IgM on the cell surface. Samples were analyzed using a MACSQuant Analyzer 16 and FlowJo v10. Graphpad Prism was used for statistics (p < 0.5).

Results: Comparable to tetanus vaccinations we saw a pronounced PC expansion on day 7 after vaccination in patients and controls. A fraction of those cells was SARS-CoV2 specific (median 7.23% (patients) vs 17.05% (controls)). Over all samples, 26.9% of these specific cells showed the expression of CD138 and HLA-DR in parallel. Additionally, we observed a bystander activation of newly generated plasmablasts and the mobilization of mature PCs that expressed only CD138 and no HLA-DR.

Conclusion: SARS-CoV-2 mRNA vaccine leads to the generation of specific PCs in healthy controls and, reduced, in patients with CID. Some of these cells exhibit a phenotype of both activation and maturation comparable to cells found in active COVID-19. Additionally, SARS-CoV-2 vaccination mobilizes mature PCs of other specificities from the bone marrow which leads to the conclusion that SARS-CoV-2-specific PCs are able to mobilize cells from survival niches and become long-lived themselves.

Disclosures: Ciripoi, Maria S.: keine; Geisen, Ulf M.: keine; Vullriede, Lena: keine; Reid, Hayley M: keine; Berner, Dennis K.: keine; Tran, Florian: keine, Sümbül, Melike: keine; Schaffarzyk, Annika: keine; Schirmer, Jan H.: keine; Zeuner, Rainald: keine; Friedrichs, Anette: keine; Steinbach, Andrea: keine; Gerdes, Sascha: keine; Bacher, Petra: keine; Schreiber, Stefan: Pfizer; Hoyer, Bimba F.: Pfizer