Artikel
Altered expression of C3a and C5a receptors in patients with rheumatic diseases
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Veröffentlicht: | 29. August 2016 |
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Gliederung
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Background: The complement system has multiple functions in the immune response such as the regulation of inflammatory mechanisms. Abnormalities of the complement system are known to play important roles in the pathogenesis of various rheumatic diseases. However, it remains to be determined if the expression of the complement receptors C3aR and C5aR by peripheral blood leukocytes (PBL) subpopulations is altered in systemic lupus erythematosus (SLE), systemic sclerosis (SSc), granulomatosis with polyangiitis (GPA) and rheumatoid arthritis (RA). The aim of this study was to assess the expression of C3aR and C5aR on peripheral blood leukocyte (PBL) subpopulations such as T helper (CD3+CD4+), T cytotoxic cells (CD3+CD8+), B cells (CD19+), neutrophils (CD15+), and monocytes (CD14+) from patients with SLE, SSc, GPA and RA in comparison to healthy controls (HC).
Methods: After surface staining using anti-CD3, -CD4, -CD8, -CD19, -CD15, -CD14, -C3aR and -C5aR fluorochrome-conjugated antibodies the expression of C3aR and C5aR on PBLs subpopulations from patients (SLE= 11, SSC=14, RA=11 and GPA=9) and healthy controls (HC=11) was analysed by flow cytometry.
Results: SLE patients showed reduced expression of C3aR on T helper cells (p = 0.0338) and neutrophils (p = 0.0364) as well as reduced C5aR expression on B cells (p = 0.0455). In turn, SSc patients demonstrated reduced C3aR expression on neutrophils (p = 0.0328), while RA patients displayed elevated C3aR expression on monocytes (p = 0.0178) and GPA patients presented elevated expression of both C3aR (p = 0.0142) and C5aR (p = 0.0302) on B cells.
Conclusion: Altered C3aR and C5aR expression patterns on PBL subpopulations were detected in different rheumatic diseases. The mechanisms behind these alterations as well as the functional consequences of abnormal C3aR and C5aR expressions need to be further investigated.