gms | German Medical Science

44. Kongress der Deutschen Gesellschaft für Rheumatologie, 30. Jahrestagung der Deutschen Gesellschaft für Orthopädische Rheumatologie, 26. Jahrestagung der Gesellschaft für Kinder- und Jugendrheumatologie

31.08. - 03.09.2016, Frankfurt am Main

Effects of amino-PVA-coated nanoparticles on CD4+ T cell activity

Meeting Abstract

  • Cindy Strehl - Charité - Universitätsmedizin Berlin, Medizinische Klinik mit Schwerpunkt Rheumatologie und klinische Immunologie, Berlin
  • Lionel Maurizi - École polytechnique fédérale de Lausanne EPFL, Institute of Materials Powder Technology Laboratory, Lausanne, Switzerland
  • Thomas Häupl - Charité - Universitätsmedizin Berlin, Medizinische Klinik mit Schwerpunkt Rheumatologie und klinische Immunologie, Berlin
  • Heinrich Hofmann - École polytechnique fédérale de Lausanne EPFL, Institute of Materials Powder Technology Laboratory, Lausanne, Switzerland
  • Frank Buttgereit - Charité - Universitätsmedizin Berlin, Medizinische Klinik mit Schwerpunkt Rheumatologie und klinische Immunologie, Berlin
  • Timo Gaber - Charité - Universitätsmedizin Berlin, Medizinische Klinik mit Schwerpunkt Rheumatologie und klinische Immunologie, Berlin

Deutsche Gesellschaft für Rheumatologie. Deutsche Gesellschaft für Orthopädische Rheumatologie. Gesellschaft für Kinder- und Jugendrheumatologie. 44. Kongress der Deutschen Gesellschaft für Rheumatologie (DGRh); 30. Jahrestagung der Deutschen Gesellschaft für Orthopädische Rheumatologie (DGORh); 26. Jahrestagung der Gesellschaft für Kinder- und Jugendrheumatologie (GKJR). Frankfurt am Main, 31.08.-03.09.2016. Düsseldorf: German Medical Science GMS Publishing House; 2016. DocER.04

doi: 10.3205/16dgrh046, urn:nbn:de:0183-16dgrh0461

Veröffentlicht: 29. August 2016

© 2016 Strehl et al.
Dieser Artikel ist ein Open-Access-Artikel und steht unter den Lizenzbedingungen der Creative Commons Attribution 4.0 License (Namensnennung). Lizenz-Angaben siehe http://creativecommons.org/licenses/by/4.0/.


Gliederung

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Background: In medical applications nanotechnology provides new opportunities for diagnostic and therapeutic interventions in a variety of human diseases. Superparamagnetic iron oxide nanoparticles (SPION) are used as high-sensitive enhancer for magnetic resonance imaging, where they represent a promising tool for early diagnosis of destructive diseases such as rheumatoid arthritis (RA) and osteoarthritis (OA).

However, safety aspects still represent crucial problems for the further development of nanotechnology based products. Therefore, the focus of our work here was to identify putative effects of amino-polyvinyl alcohol-coated (a-PVA) SPION on human immune cell functions. Since we could demonstrate in former studies that professional phagocytes are activated by a-PVA-SPION we here focused on the influence of these nanoparticles on human T helper cell activity.

Methods: PBMCs were isolated from blood samples obtained from healthy donors (HD) or patients suffering from RA. Primary human CD4 positive T cells were separated via MACS-Sort and incubated with the mitogen PHA-L (5µg/ml) and/or varying doses of a-PVA-SPION (1 µg/ml, 10 µg/ml, and 100 µg/ml) or left untreated for 20 h (analysis of caspase-3/7-activity, intracellular ATP content and CD25 expression) or 72 h (analysis of proliferation and CD25 expression). Cells were incubated under either normoxia (app. 18%O2) or hypoxia (1%O2) in order to mimic conditions found in the circulation and in the inflamed joint, respectively.

Results: We observed for PHA-L / a-PVA-SPION co-stimulated T cells from HD a decrease in cell count whereas the caspase-3/7-activity is increased in these samples, as expected. Although, we observed that T cells from RA patients are more susceptible to low-dose a-PVA-SPION-induced apoptosis than T cells from HD, in both groups a-PVA-SPION do not activate CD4+ T cells per se and do not influence mitogen-mediated T cells activation with regard to CD25 expression and cell proliferation. Nevertheless, we were able to demonstrate that CD4+ T cells obtained from RA patients and healthy subjects differ in their response to mitogen stimulation and oxygen availability.

Conclusion: PVA-SPION at concentrations up to 100µg/ml do neither activate nor significantly influence mitogen-stimulated CD4+ T cells activation and have negligible influence on T cells apoptosis.