Artikel
Photosynthetic Stem Cell Therapy for Tissue Regeneration
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Veröffentlicht: | 16. August 2017 |
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Gliederung
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Introduction: A frequent barrier to overcome for Tissue Engineering scaffolds for regenerating tissue defects is ensuring a proper amount of oxygen, because of the delay in their vascularization and the rely on diffusion to deliver oxygen throughout the scaffold.
Methods and results: Exploiting the photosynthesis process, our team developed the first generation of photosynthetic biomaterials, which in the presence of light decrease tissue hypoxia by the constant supply of photosynthetic oxygen, allowing tissue oxygenation in the absence of environmental or vascular oxygen supply. Here we propose an innovative strategy which exploits the trophic capacity of human Adipose-Derived Mesenchymal Stem Cells (ASC) with microalgae Chlamydomonas reinhardtii cells to provide a local oxygen source of oxygen independently of blood perfusion, therefore improving the regenerative potential of biomaterials by the local and constant release of paracrine derived stem cell factors and oxygen.
Briefly, we optimized the best co-culture conditions for the ASCs and C. reinhardtti in scaffolds for dermal regeneration ensuring basic parameters like morphology and viability are not being affected, and then characterized the release of paracrine-derived stem cell factors and photosynthetic oxygen from seeded biomaterials in vitro. We also performed excisional wounds in a preclinicalin vivomodel to evaluate the seeded biomaterials and afterwards were able to regrow the implanted cells out of biopsies of the explanted scaffolds, ensuring the cells remained viable.
Conclusion: This approach represents a new concept in regenerative medicine that can be easily translated to its clinical use, and with promising advantages for the development of further Tissue Engineering applications.