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73. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie (DGNC)
Joint Meeting mit der Griechischen Gesellschaft für Neurochirurgie

Deutsche Gesellschaft für Neurochirurgie (DGNC) e. V.

29.05. - 01.06.2022, Köln

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An implanted spinal window chamber allows for chronic longitudinal in vivo microscopy following experimental spinal cord injury in the mouse

Chronische longitudinale in-vivo-Mikroskopie mittels implantierter Rückenmarkskammer nach experimenteller traumatischer Rückenmarksschädigung in der Maus

Meeting Abstract

Suche in Medline nach

  • presenting/speaker Laurens Roolfs - Charité – Universitätsmedizin Berlin, Neurochirurgie und experimentelle Neurochirurgie, Berlin, Deutschland
  • Nima Taheri - Charité – Universitätsmedizin Berlin, Neurochirurgie und experimentelle Neurochirurgie, Berlin, Deutschland
  • Lea Meyer - Charité – Universitätsmedizin Berlin, Neurochirurgie und experimentelle Neurochirurgie, Berlin, Deutschland
  • Lilly Waldmann - Charité – Universitätsmedizin Berlin, Neurochirurgie und experimentelle Neurochirurgie, Berlin, Deutschland
  • Katharina Kersting - Charité – Universitätsmedizin Berlin, Neurochirurgie und experimentelle Neurochirurgie, Berlin, Deutschland
  • Melina Nieminen-Kelhä - Charité – Universitätsmedizin Berlin, Neurochirurgie und experimentelle Neurochirurgie, Berlin, Deutschland
  • Kim Cornelia Fischer - Charité – Universitätsmedizin Berlin, Neurochirurgie und experimentelle Neurochirurgie, Berlin, Deutschland
  • Christian Uhl - Charité – Universitätsmedizin Berlin, Neurochirurgie und experimentelle Neurochirurgie, Berlin, Deutschland
  • Jan-Erik Ode - Charité – Universitätsmedizin Berlin, Wissenschaftliche Werkstätten, Berlin, Deutschland
  • Andre Rex - Charité – Universitätsmedizin Berlin, Experimentelle Neurologie, Berlin, Deutschland
  • Michael Fehlings - Toronto Western Hospital, University Health Network, Neurosurgery, Toronto, Kanada
  • Peter Vajkoczy - Charité – Universitätsmedizin Berlin, Neurochirurgie und experimentelle Neurochirurgie, Berlin, Deutschland
  • Vanessa Hubertus - Charité – Universitätsmedizin Berlin, Neurochirurgie und experimentelle Neurochirurgie, Berlin, Deutschland

Deutsche Gesellschaft für Neurochirurgie. 73. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie (DGNC), Joint Meeting mit der Griechischen Gesellschaft für Neurochirurgie. Köln, 29.05.-01.06.2022. Düsseldorf: German Medical Science GMS Publishing House; 2022. DocP193

doi: 10.3205/22dgnc508, urn:nbn:de:0183-22dgnc5085

Veröffentlicht: 25. Mai 2022

© 2022 Roolfs et al.
Dieser Artikel ist ein Open-Access-Artikel und steht unter den Lizenzbedingungen der Creative Commons Attribution 4.0 License (Namensnennung). Lizenz-Angaben siehe http://creativecommons.org/licenses/by/4.0/.

Gliederung

Text

Objective: Longitudinal in vivo microscopy allows for a direct real-time insight into pathophysiological processes in the living organism. After experimental Spinal Cord Injury (SCI) it can contribute to a deeper understanding of pathophysiological processes and potential therapeutic approaches. The implantation of a spinal window chamber enables longitudinal in vivo imaging of the spinal cord without the necessity for repeated surgery. With this study, we show the modification of an established spinal window chamber for chronic longitudinal in vivo imaging after experimental SCI in the mouse.

Methods: Adult C57BL/6J mice (m/f, n=8) underwent low-thoracic (Th11/12) clip-compression SCI using a modified aneurysm clip (5g, 60s) or sham-injury (two-level-laminectomy), followed by the implantation of a spinal window chamber for later in vivo microscopy. Perioperative antibiotic therapy with amoxicillin and postoperative pain medication with buprenorphine was applied. In vivo white light and fluorescence microscopy to image blood flow (FITC) and inflammation (Rhodamine-dextran) was performed at postoperative days 1, 3, 7, 14 and 28. Additionally, neurobehavioral assessment (Catwalk® automated gait analysis and Basso Mouse Scale) was performed at the given time points. Neurobehavioral analysis was compared to specimens (C57BL/6J mice, m/f, n=12) with SCI or sham-injury without spinal window chamber implantation. Histological analysis for tissue integrity assessment (Luxol-Fast-Blue + H&E) was performed after 28 days follow-up.

Results: Chronic longitudinal in vivo white light and fluorescence microscopy was possible up to 28 days postoperatively after both SCI and sham injury. Only mild inflammation was observed in individual specimens. Specimens with an implanted spinal window chamber did not show differences in automated gait analysis (Catwalk®), Basso Mouse Scale, postoperative body weight development or histologically assessed spinal cord integrity compared with specimens which received the same surgery without spinal window chamber implantation.

Conclusion: The implantation of a spinal window chamber after clip compression SCI in the mouse is feasible and allows for longitudinal in vivo imaging up to the chronic injury phase without modifying neurological function or spinal cord integrity compared to specimens without chamber implantation. To achieve more detailed in vivo imaging at a greater depth, longitudinal two-photon microscopy can be integrated.