Artikel
Ethanol-induced inflammation and aging in endothelial cells was reduced by Doxycycline treatment
Doxycyclin reduziert Ethanol-induzierte Entzündung und Alterung in Endothelzellen
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Autoren
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Xuanchen Li
- Universitätsklinikum Düsseldorf, Klinik für Neurochirurgie, Düsseldorf, Deutschland
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Dilaware Khan
- Universitätsklinikum Düsseldorf, Klinik für Neurochirurgie, Düsseldorf, Deutschland
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Huakang Zhou
- Universitätsklinikum Düsseldorf, Klinik für Neurochirurgie, Düsseldorf, Deutschland
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Norbert Gerdes
- Universitätsklinikum Düsseldorf, Department of Cardiology, Pulmonology, and Vascular Medicine, Düsseldorf, Deutschland
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Daniel Hänggi
- Universitätsklinikum Düsseldorf, Klinik für Neurochirurgie, Düsseldorf, Deutschland
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Sajjad Muhammad
- Universitätsklinikum Düsseldorf, Klinik für Neurochirurgie, Düsseldorf, Deutschland
| Veröffentlicht: | 25. Mai 2022 |
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Gliederung
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Objective: Hypertension, smoking and excess alcohol consumption are associated with the development of aortic and intracranial aneurysms (ICAs). Endothelial dysfunction is considered as one of the important mechanisms involved in aneurysm pathophysiology. Here, we investigated the effects of ethanol on human umbilical vein endothelial cells (HUVECs) and screened 171 drugs to find drug candidates to reverse alcohol mediated changes in endothelial cells.
Methods: HUVECs (Promocell, Heidelberg, Germany) were treated with different concentrations (300mM, 400mM) of Ethanol with or without Doxycycline (2µg/mL). Telomere length was quantified as telomere to single-copy gene (TS) ratio. Telomere length and the mRNA expression were quantified by qRT-PCR and protein level was analyzed by Western blot. All experiments were performed with three biological replicates and each biological replicate had technical triplicates. For statistical analysis, the Student t-test was applied for two groups and one-way ANOVA to compare more than two groups.
Results: Ethanol treatment accelerated cellular aging in HUVECs which was observed through shortening of telomere length (Control=1±0.08, Ethanol=0.26±0.04) and relative mRNA expression of aging marker P21 (Control=1±0.08, Ethanol=2.09±0.07). After Doxycycline treatment the telomere length was recovered (Control=1±0.08, Ethanol=0.26±0.04 Ethanol + Doxycycline=0.68±0.11), Lamin b1 protein (marker for aging) expression was increased (Control=1.18±0.09, Ethanol=0.65±0.05, Ethanol + Doxycycline=1±0.1), and relative mRNA expression of P21 was reduced (Control=1±0.11, Ethanol=2.09±0.07 Ethanol + Doxycycline=1.9±0.01). Doxycycline reduced the mRNA expression of inflammatory markers (MCP-1: Control=1±0.17, Ethanol=9.28±0.12, Ethanol + Doxycycline=6.68±.041; IL-17: Control=1±0.12, Ethanol=8.15±1.15, Ethanol + Dox=4.9±0.86), endothelial dysfunction marker ICAM-1 (Control=1±0.15, Ethanol=3.6±0.21, Ethanol + Doxycycline=1.7±0.28) and MMP2 (Control=1±0.27, Ethanol=1.89±0.16, Ethanol + Doxycycline=1.29±0.13). All values are mean±StDev and P<0.05.
Conclusion: Ethanol enhanced cellular aging in endothelial cells and caused endothelial dysfunction. Doxycycline reduced accelerated aging and decreased endothelial dysfunction. Doxycycline can be a candidate drug for endothelial cell dysfunction-associated diseases including ICAs.
