Artikel
Neointima sealing provided by endothelial cells of the parent artery in a rat saccular side wall model – methodology of in-vivo cell tracer injection
Neointima Ausheilung vermittelt durch endotheliale Zellen des adjacent vessels in einem Seitenwandmodell der Ratte – Methodologie einer in vivo-cell Tracer Verabreichung
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Autoren
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Stefan Wanderer
- Kantonsspital Aarau, Klinik für Neurochirurgie, Aarau, Schweiz
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Basil Grüter
- Kantonsspital Aarau, Klinik für Neurochirurgie, Aarau, Schweiz
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Jeannine Rey
- Kantonsspital Aarau, Klinik für Neurochirurgie, Aarau, Schweiz
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Gwendoline Boillat
- Kantonsspital Aarau, Klinik für Neurochirurgie, Aarau, Schweiz
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Sivani Sivanrupan
- Inselspital, Universitätsspital Bern, Cerebrovascular Research Group, Department for BioMedical Research, Bern, Schweiz
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Kristina Catalano
- Inselspital, Universitätsspital Bern, Cerebrovascular Research Group, Department for BioMedical Research, Bern, Schweiz
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Michael von Gunten
- Institute of Pathology Laenggasse, Bern, Schweiz
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Hans Rudolf Widmer
- Neurocenter and Regenerative Neuroscience Cluster, Department of Neurosurgery, Neurocenter and Regenerative Neuroscience Cluster, Bern, Schweiz
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Serge Marbacher
- Kantonsspital Aarau, Klinik für Neurochirurgie, Aarau, Schweiz
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Lukas Andereggen
- Kantonsspital Aarau, Klinik für Neurochirurgie, Aarau, Schweiz
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Philipp J. Jost
- Kantonsspital Aarau, Klinik für Neurochirurgie, Aarau, Schweiz
| Veröffentlicht: | 25. Mai 2022 |
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Gliederung
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Objective: Microsurgical clipping creates a subsequent barrier of blood flow into intracranial aneurysms, whereas endovascular treatment relies on neointima and thrombus formation. The source of endothelial cells covering the endoluminal layer of the neointima remains unclear. The aim of the present study was to investigate this origin after cell-tracer injection in the Helsinki rat microsurgical sidewall aneurysm model.
Methods: Sidewall aneurysms were created suturing decellularized or vital arterial pouches end-to-side to the abdominal part of the aorta in male Lewis rats. Prior arteriotomy and aneurysm suture, cell-tracer injection was performed into the clamped aorta to lable endothelial cells in the adjacent vessel and enable tracking their proliferation during follow-up. Treatment followed by coil- (n = 16) or stent-embolization (n = 15). At follow-up (7 days or 21 days), all rats underwent fluorescence angiography followed by aneurysm harvesting, macroscopic and histological evaluation with immunohistological cell counts for specific regions of interest.
Results: None of the 31 aneurysms had ruptured upon follow-up. 4 animals died prematurely. Macroscopical residual perfusion was observed in 75.0% coiled and 7.0% of stented rats. The amount of cell-tracer positive cells was significantly elevated in decellularized stented compared to coiled aneurysms regarding thrombus on day 7 (p = 0.01) and neointima on day 21 (p = 0.04); in vital aneurysms no significant differences in thrombus or neointima were found.
Conclusion: Our findings confirm worse healing patterns in coiled compared to stented aneurysms. Neointima formation seems particularly dependent on the parent artery in decellularized aneurysms, whereas it is supported by the recruitment from aneurysm wall cells in vital cell-rich walls. In terms of translation, stent-treatment might be more sufficient for highly degenerated aneurysms whereas coiling alone might be adequate for aneurysms with mostly healthy vessel walls.
