Artikel
Meta review on perforation model of subarachnoid haemorrhage in mice – filament material as a moderator of mortality
Meta-Review zum Perforationsmodell der Subarachnoidalblutung bei Mäusen: Filamentmaterial als Moderator der Mortalität
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Veröffentlicht: | 25. Mai 2022 |
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Gliederung
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Objective: Since description of the first animal model of subarachnoid haemorrhage (SAH) in 1979, researchers have improved the technique and procedure to increase suitability for translational research. Here we focus on the reported experimental procedure of the perforation SAH model in mice and aim to identify predicting factors for mortality, severity of SAH grade and frequency of large artery vasospasm.
Methods: We conducted a systemic review on studies using SAH perforation model in Wild Type C57BL/6 mice. A set of twenty-two different model characteristics including outcome, mouse strain, surgical procedure, filament material and anaesthetic specifications were extracted from each article. Afterwards, a random-effects meta-analysis was used to identify variances in mortality, SAH severity grade, large artery vasospasm and experimental moderators of it, provided enough articles reported that particular parameter.
Results: Of our 430 articles, 42 were eligible for the meta-analysis. Certain SAH perforation model characteristics were insufficently reported, e.g., location of perforation (not reported in n = 6 articles), postsurgical pain management (n = 32), filament material (n = 15), age (n = 10) and weight of mice (n = 10). A large diversity in reporting was observed throughout articles in terms of injective anaesthetics and the location of the perforation. Compared to Cisterna Magna blood injection model (0 - 16 % mortality) (Leclerc et al. 2018) the overall animal mortality was 20.8 % [95 %-CI: 16.8 %, 25.5 %] following a median observation time of three days after SAH induction. The mortality increased with longer observational period (0.9 % increase per additional day, p = 0.038). Interestingly, after removing four hyper acute studies for pathophysiological mechanisms (time < 1 day), the material of the filament used for perforation significantly correlated with animal mortality (p = 0.008). The mean SAH grade, reported in 16 articles, was 10.8 [9.7, 11.9] on the scale described by Sugawara (2008). Furthermore, the mean diameter of large arteries was 26.1 % [3.3 %, 48.9 %] smaller after SAH compared to the diameter of sham operated control groups.
Conclusion: Reporting quality of experimental procedures and outcome in perforation mouse model of SAH is poor. Uniform standards of experimental procedures might help to increase overall comparability. In this context, filament material probably influences mortality and may oppose to reduce needed number of animals.