Artikel
Development and characterisation of a dual saccular elastase-digested aneurysm rabbit model
Entwicklung und Charakterisierung eines doppelten sacculären Elastase-degenerierten Aneurysma Kaninchenmodells
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Veröffentlicht: | 4. Juni 2021 |
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Gliederung
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Objective: Intracranial aneurysm rupture is a devastating condition leading to high mortality and morbidity. The development of preclinical animal models with hemodynamic, morphologic and histologic characteristics close to humans one plays a key role in our understanding of the pathophysiological processes and in the development of new therapeutic strategies for treatment of intracranial aneurysm. This study aims to describe a new rabbit aneurysm model with different hemodynamic conditions, i.e. stump and bifurcation aneurysms, both with degenerated walls condition, in the same animal.
Methods: Five female New Zealand white rabbits with a mean weight of 4.0 (± 0.3) kg and mean age of 25 (±5) weeks underwent microsurgical stump and bifurcation aneurysm creation. Stump aneurysm was created by right common carotid artery (CCA) exposure at its origin at the brachiocephalic trunk. After temporary clip application at the CCA origin and 2cm above, the segment was treated with a local injection of 100 U Elastase for 20 minutes. Bifurcation aneurysm was created by suturing an elastase-treated arterial pouch into the end-to-side anastomosis of the right CCA to left CCA. Patency was controlled intraoperatively by fluorescence angiography and at follow up after 24±2 days by magnetic reasonnance angiography and macroscopic tissue inspection.
Results: The average duration of surgery was 221 minutes. The creation of two aneurysms in the same animal was successful in all rabbits. We observed no mortality during surgery and follow-up. One rabbit experienced postoperative complication with a vestibular syndrome, which recovered spontaneously within one day. All aneurysms were patent immediately after creation except for one bifurcation aneurysm which showed an extreme tissue reaction due to elastase incubation and an immediate thrombosis. Except this one, all aneurysms were patent at follow up.
Conclusion: We demonstrate for the first time the feasibility of creating a two-aneurysms rabbit model with stump and bifurcation hemodynamic characteristics and highly degenerated wall conditions. This model allows to study the natural course and potential treatment strategies on the basis of aneurysm biology and under consideration of different flow conditions.