gms | German Medical Science

72. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie (DGNC)
Joint Meeting mit der Polnischen Gesellschaft für Neurochirurgie

Deutsche Gesellschaft für Neurochirurgie (DGNC) e. V.

06.06. - 09.06.2021

Collagen labelling for visualisation of structural instability in intracranial aneurysms

Kollagenmarkierung zur Darstellung der strukturellen Stabilität von intrakraniellen Aneurysmen

Meeting Abstract

  • presenting/speaker Katharina Hackenberg - Universitätsmedizin Mannheim, Neurochirurgie, Mannheim, Deutschland
  • Nicola Willett - Universitätsmedizin Mannheim, Neurochirurgie, Mannheim, Deutschland
  • Amr Abdulazim - Universitätsmedizin Mannheim, Neurochirurgie, Mannheim, Deutschland
  • Rita Dreier - Universitätsklinikum Münster, Institut für Physiologische Chemie und Pathobiochemie, Münster, Deutschland
  • Daniel Hänggi - Universitätsklinikum Düsseldorf, Klinik für Neurochirurgie, Düsseldorf, Deutschland
  • Björn Wängler - Universitätsmedizin Mannheim, Institut für Klinische Radiologie und Nuklearmedizin, Mannheim, Deutschland
  • Carmen Wängler - Universitätsmedizin Mannheim, Institut für Klinische Radiologie und Nuklearmedizin, Mannheim, Deutschland
  • Nima Etminan - Universitätsmedizin Mannheim, Neurochirurgie, Mannheim, Deutschland

Deutsche Gesellschaft für Neurochirurgie. 72. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie (DGNC), Joint Meeting mit der Polnischen Gesellschaft für Neurochirurgie. sine loco [digital], 06.-09.06.2021. Düsseldorf: German Medical Science GMS Publishing House; 2021. DocP160

doi: 10.3205/21dgnc442, urn:nbn:de:0183-21dgnc4425

Veröffentlicht: 4. Juni 2021

© 2021 Hackenberg et al.
Dieser Artikel ist ein Open-Access-Artikel und steht unter den Lizenzbedingungen der Creative Commons Attribution 4.0 License (Namensnennung). Lizenz-Angaben siehe http://creativecommons.org/licenses/by/4.0/.


Gliederung

Text

Objective: The main molecular constituent in the wall of intracranial aneurysms (IA) is collagen type I, which cannot be illustrated by existing imaging modalities. Ongoing wall remodelling in IA, especially molecular turnover with synthesis of immature/structural deficient collagen type I, seems to be associated with IA instability. At present the estimation of the individual IA instability, i.e. the rupture risk, remains challenging. Therefore, we developed a novel Positron Emission Tomography contrast agent to visualize immature/novel collagen in human IA as a possible indicator for instability.

Methods: IA samples derived from patients undergoing surgical IA repair, were used for radio- and immunolabelling as well as histological staining. Longitudinal cryosections of the IA including the dome and transition zone (between dome and neck) were performed. 12µm cryosections were incubated with the tracer 68Ga-NODAGA-Collagelin and visualised by autoradiography. 9µm cryosections were used for immunolabelling of collagen alpha 1 chain type I and visualized by confocal laser microscopy. 9µm cryosections were used for histological staining and visualised by light microscopy. Immature and mature collagen was differentiated by polarisation microscopy. Qualitative assessment was performed by two independent investigators.

Results: Between 6/2016 and 11/2019 25 IA samples (11 unruptured, 14 ruptured) from 25 IA patients (18 female, 6 male) with 57.4±9.9 years were included. Polarisation microscopy demonstrated regions of mature collagen, which were localised independent of the IA region (neck to dome), but regions of immature collagen were predominantly found in IA domes. Radiolabelling demonstrated increased uptake in IA regions with immature collagen and less uptake in regions with mature IA collagen. Figures 1 [Fig. 1] and Figure 2 [Fig. 2] serve as illustrative examples for an unruptured and a ruptured IA.

Conclusion: Our novel collagen radiotracer seems to specifically label human IA regions with immature/structurally deficient collagen and could serve as a novel radiological indicator for molecular IA stability. To further facilitate clinical use, we will corroborate our current findings on collagen imaging in vivo after induction of IA in an animal model by autoradiography and PET.