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72. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie (DGNC)
Joint Meeting mit der Polnischen Gesellschaft für Neurochirurgie

Deutsche Gesellschaft für Neurochirurgie (DGNC) e. V.

06.06. - 09.06.2021

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Synergistic effect of 5-amino-levulinic acid and gold nanoparticles in photodynamic therapy of immortalised human vestibular schwannoma cells

Synergistischer Effekt von 5-Aminolävulinsäure und Goldnanopartikeln bei der photodynamischen Therapie von immortalisierten humanen Vestibularisschwannom-Zellen

Meeting Abstract

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  • presenting/speaker Cihat Karadag - Universitätsklinikum Düsseldorf, Neurochirurgische Klinik, Düsseldorf, Deutschland
  • Hannah H. Gull - Universitätsklinikum Düsseldorf, Neurochirurgische Klinik, Düsseldorf, Deutschland
  • Ulf Dietrich Kahlert - Universitätsklinikum Düsseldorf, Neurochirurgische Klinik, Düsseldorf, Deutschland
  • Daniel Hänggi - Universitätsklinikum Düsseldorf, Neurochirurgische Klinik, Düsseldorf, Deutschland
  • Jan Frederick Cornelius - Universitätsklinikum Düsseldorf, Neurochirurgische Klinik, Düsseldorf, Deutschland

Deutsche Gesellschaft für Neurochirurgie. 72. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie (DGNC), Joint Meeting mit der Polnischen Gesellschaft für Neurochirurgie. sine loco [digital], 06.-09.06.2021. Düsseldorf: German Medical Science GMS Publishing House; 2021. DocP052

doi: 10.3205/21dgnc340, urn:nbn:de:0183-21dgnc3405

Veröffentlicht: 4. Juni 2021

© 2021 Karadag et al.
Dieser Artikel ist ein Open-Access-Artikel und steht unter den Lizenzbedingungen der Creative Commons Attribution 4.0 License (Namensnennung). Lizenz-Angaben siehe http://creativecommons.org/licenses/by/4.0/.

Gliederung

Text

Objective: Vestibular schwannoma (VS) is a common benign brain tumor. Current management includes wait and scan, surgery or stereotactic irradiation or a combination. Photo-dynamic therapy (PDT) was shown to be effective in a number of tumors. In order to explore novel adjuvant treatment strategies, we sought to investigate PDT in VS. Therefore, we tested PDT based on 5-ALA in the immortalized human VS cell line HEI-193. Furthermore, we analyzed if PDT effectiveness may be enhanced by gold nanoparticles (GNP) as observed previously.

Methods: For PDT treatment, cells were seeded in 96-well plates. Three drug constellations were tested: 5-ALA only, GNP only and a combination of both. After reaching adherence, cells were incubated for 6 h with different concentrations of gold nanoparticles (20 – 50 μg/ml, group I) or 5-ALA (20 – 50 μg/ml, group II), respectively. In group III, cells were first incubated for 6h with GNP and then 6h with 5-ALA at varying doses as in the other groups. Afterwards cells were irradiated by a diode laser for 625 seconds at λ=635 nm with 15.6 J/cm2. Cell viability was assessed by WST-1 assay. Negative controls were also performed.

Results: Both drugs and their combination induced a dose-dependent lethal PDT effect, respectively. The strongest effect was observed for gold nanoparticles plus 5-ALA as compared to 5-ALA or GNP only (p < 0.01).

Conclusion: Gold nanoparticles and 5-ALA showed a synergistic effect in PDT of immortalized human vestibular schwannoma cells as compared to the effect of each photosensitizer alone. Based on this preliminary in vitro study further testing on primary cell cultures is warranted.