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72. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie (DGNC)
Joint Meeting mit der Polnischen Gesellschaft für Neurochirurgie

Deutsche Gesellschaft für Neurochirurgie (DGNC) e. V.

06.06. - 09.06.2021

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Ultrasound-induced drug release from nimodipine-loaded nanoparticles – in-vivo analysis using the chicken chorioallantoic membrane model

Ultraschall-induzierte Medikamentenfreisetzung von mit Nimodipin geladenen Nanopartikel – in vivo Analysen am CAM-Modell

Meeting Abstract

Suche in Medline nach

  • presenting/speaker Katja Döring - Universitätsmedizin Göttingen, Neuroradiologie, Göttingen, Deutschland
  • Henning Schroeder - Universitätsmedizin Göttingen, German Center for Neurodegenerative Diseases, Göttingen, Deutschland
  • Swetlana Sperling - Universitätsmedizin Göttingen, Neurochirurgie, Göttingen, Deutschland
  • Milena Ninkovic - Universitätsmedizin Göttingen, Neurochirurgie, Göttingen, Deutschland
  • Christine Stadelmann - Universitätsmedizin Göttingen, Neuropathologie, Göttingen, Deutschland
  • Frank Streit - Universitätsmedizin Göttingen, Klinische Chemie, Göttingen, Deutschland
  • Lutz Binder - Universitätsmedizin Göttingen, Klinische Chemie, Göttingen, Deutschland
  • Veit Rohde - Universitätsmedizin Göttingen, Neurochirurgie, Göttingen, Deutschland
  • Vesna Malinova - Universitätsmedizin Göttingen, Neurochirurgie, Göttingen, Deutschland

Deutsche Gesellschaft für Neurochirurgie. 72. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie (DGNC), Joint Meeting mit der Polnischen Gesellschaft für Neurochirurgie. sine loco [digital], 06.-09.06.2021. Düsseldorf: German Medical Science GMS Publishing House; 2021. DocV300

doi: 10.3205/21dgnc284, urn:nbn:de:0183-21dgnc2842

Veröffentlicht: 4. Juni 2021

© 2021 Döring et al.
Dieser Artikel ist ein Open-Access-Artikel und steht unter den Lizenzbedingungen der Creative Commons Attribution 4.0 License (Namensnennung). Lizenz-Angaben siehe http://creativecommons.org/licenses/by/4.0/.

Gliederung

Text

Objective: Nimodipine has been demonstrated to reduce ischemic complications following subarachnoid hemorrhage (SAH). In order to overcome the fist-pass effect of nimodipine, nanocarrier systems with sustained local drug delivery have gained increasing interest. The aim of this study is to produce nimodipine-loaded polymers and to evaluate the possibility of controlled drug release by applying ultrasound in the chicken chorioallantoic membrane (CAM) model.

Methods: Nimodipine-loaded nanoparticles were produced with Pluronic® F127 by the direct dissolution method. Vasospasm was induced by direct ultrasound-application (Physioson, 3 MHz, 1.0 W/cm2) for 60 seconds. The drug release from was performed by means of ultrasound (1 MHz, 1.7 W/cm²). The effect on the vessels was evaluated with the CAM-model. Nimodipine-loaded nanoparticles were applied in the control group without and in the treatment group with ultrasound application. The vessel diameter before and after treatment was visualized and measured using ImageJ.

Results: The ultrasound-induced nimodipine-release was evaluated on 20 embryo eggs (10 treatment group, 10 control). We found no change in the vessel diameter over time in the control group. The mean relative reduction in vessel diameter after vasospasm induction was 46% (range 44-56%). Eight minutes after ultrasound-induced nimodipine-release in the treatment group the mean vessel diameter of spastic vessels increased again to 90% (range 83-91%) of the initial diameter, which was statistically significant (t-test, p=0.0002).

Conclusion: A controlled drug release from nimodipine-loaded nanoparticles could be reliably induced by applying low frequency continuous wave ultrasound, which resulted in a significant vasospasm reduction in the CAM-model. These results are encouraging further evaluation of this treatment concept with intrathecal application of nimodipine-loaded nanoparticles in an animal SAH-model for controlled vasospasm resolution.