gms | German Medical Science

Objective: In a previous study, we investigated the influence of glycation in invasiveness on two meningioma cell lines representing the WHO grade I and III. Therefore cells were treated with Methylglyoxal (MGO). We could show, that glycation leads to a more aggressive behavior of the beningn cell line and to a less aggressive behavior of the malignant cell line. In this study, we wanted to investigate the molecular mechanisms of this behaviour.

Methods: Two meningioma cell lines, representing the WHO grade I (BEN-MEN-1) and the WHO grade III (IOMM-Lee) were cultured in the absence or presence of MGO. Expression of N-Cadherin, E-Cadherin, several Integrins, RAGE (Receptor for Advanced Glycation Endproducts), NCAM (neuronal cell adhesion molecul), Tn-Antigen and GALNT (GalNAc polypeptide N-acetylgalactosaminyltransferases) were analyzed.

Results: We observed that E-Cadherin expression was increased after glycation and N-Cadherin expression was decreased in BEN-MEN-1 cells. The Cadherin expression was not affected in IOMM-Lee cells. In both cell lines, we could observe an increased RAGE expression after glycation. Tn-Antigen was increased after glycation in BEN-MEN1 cells and was not affected in IOM-LEE cells after glycation. The expression of NCAM was decreases in IOM-LEE cells after glycation. For the GALNT 10 we saw no alteration in IOM-LEE cells after glycation where it was decreased in BEN-MEN1 cells.

Conclusion: Glycation of benign meningioma cell line results in an increased E-Cadherin and RAGE expression and a decreased N-Cadherin expression, which could be an evidence for more invasiveness of the benign meningioma cell line. IOMM-Lee cells were not affected in Cadherin expression through glycation. In addition, we saw changes in the expression of Tn-Antigen and GALNT 10 in BEN-MEN1 cells after glycation.

Figure 1 [Fig. 1], Figure 2 [Fig. 2]