Artikel
Fluorescent image-guided resection for intracranial meningioma with a new somatostatin-ligands-dye – an experimental study on the nude mice
Fluoreszenzgestützte Resektion intrakranieller Meningeome mit einem neuartigen Somatostatin-Liganden-Rezeptor-Farbstoff – eine Tiermodellstudie an der Nacktmaus
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Autoren
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Simon Müller
- Universitätsklinikum des Saarlandes, Klinik für Neurochirurgie, Homburg/Saar, Deutschland
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Sebastian Senger
- Universitätsklinikum des Saarlandes, Klinik für Neurochirurgie, Homburg/Saar, Deutschland
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Joachim Oertel
- Universitätsklinikum des Saarlandes, Klinik für Neurochirurgie, Homburg/Saar, Deutschland
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Stefan Linsler
- Universitätsklinikum des Saarlandes, Klinik für Neurochirurgie, Homburg/Saar, Deutschland
| Veröffentlicht: | 4. Juni 2021 |
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Gliederung
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Objective: The use of photodynamic agents in malignant cranial tumor surgery is quite common. All methods that permit intraoperative identification of residual tumor tissue may be of benefit. This study was established to investigate fluorescent image-guided resection for intracranial meningioma in the nude mice with a new designed dye.
Methods: Primary meningioma cell cultures were stereotactically implanted subdural into 20 nude mice. 90 days after inoculation of the cells, a cranial MRI with contrast material was performed for detection of tumor growth. After identification of meningioma growth in MR images, FAM-TOC5,6-Carboxyfluoresceine-Tyr3-Octreotide was injected intravenously and tumor mass was hereafter resected with fluorescent microscope and endoscope. Finally, brain dissection were performed a histologically processed for measurement of extent of resection.
Results: Tumor growth was detected in 18 of 20 mice in MRI after 90 days of inoculation. The tumor mass could be clearly identified under fluorescent view of the microscope and endoscope after injecting FAM-TOC5,6-Carboxyfluoresceine-Tyr3-Octreotide. The tumor margins could be better visualized and no remnant tumor remained in situ. The histological analysis revealed a GTR in all cases.
Conclusion: This study demonstrates the use of FAM-TOC5,6-Carboxyfluoresceine-Tyr3-Octreotide in fluorescent identification of meningioma cells in vivo for the first time. The authors were able to establish an experimental animal model for fluoesence guided meningimoa resection with a somatostatin-ligand based fluorescence dye for the first time.
