gms | German Medical Science

71. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie (DGNC)
9. Joint Meeting mit der Japanischen Gesellschaft für Neurochirurgie

Deutsche Gesellschaft für Neurochirurgie (DGNC) e. V.

21.06. - 24.06.2020

The effect of combined treatment with 5-aminolevulinic acid and X-Ray irradiation on proliferation and migration of human U-87 glioblastoma cell line

Der Effekt von kombinierter Behandlung mit 5-Aminolevulinsäure und Röntgenbestrahlung auf Proliferation und Migration von humanen U-87 Glioblastom Zelllinien

Meeting Abstract

  • presenting/speaker Maryam Khaleghi Ghadiri - Westfälische Wilhelms-Universität Münster, Neurochirurgie, Münster, Deutschland
  • Mohammad Jalili-Nik - Universitätsklinikum Münster, Klinik für Neurochirurgie, Münster, Deutschland; Ferdowsi University of Mashhad, Neuroscience, Mashhad, Iran
  • Abolfazl Maghrouni - Westfälische Wilhelms-Universität Münster, Neurochirurgie, Münster, Deutschland
  • Farzaneh Abbasinezhad Moud - Westfälische Wilhelms-Universität Münster, Neurochirurgie, Münster, Deutschland
  • Bilal Al Barim - Westfälische Wilhelms-Universität Münster, Neurochirurgie, Münster, Deutschland
  • Ali Gorji - Universitätsklinikum Münster, Klinik für Neurochirurgie, Münster, Deutschland
  • Walter Stummer - Universitätsklinikum Münster, Klinik für Neurochirurgie, Münster, Deutschland

Deutsche Gesellschaft für Neurochirurgie. 71. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie (DGNC), 9. Joint Meeting mit der Japanischen Gesellschaft für Neurochirurgie. sine loco [digital], 21.-24.06.2020. Düsseldorf: German Medical Science GMS Publishing House; 2020. DocV182

doi: 10.3205/20dgnc178, urn:nbn:de:0183-20dgnc1782

Veröffentlicht: 26. Juni 2020

© 2020 Khaleghi Ghadiri et al.
Dieser Artikel ist ein Open-Access-Artikel und steht unter den Lizenzbedingungen der Creative Commons Attribution 4.0 License (Namensnennung). Lizenz-Angaben siehe http://creativecommons.org/licenses/by/4.0/.


Gliederung

Text

Objective: 5-Aminolevulinic acid (5-ALA)-induced fluorescence guidance for surgical treatment has been shown to improve overall survival in patients with glioblastoma (GBM). The aim of this study was to investigate the potential cytotoxic effects of combined treatment with 5-ALA and X-ray irradiation on the viability and migration of human U87 GBM cells.

Methods: The U87 human GBM cells were treated with various concentrations of 5-ALA (100 and 300 µg/ml) with and without X-ray irradiation (2-14 Gy) and the cell viability and migration was assessed after 10 days.

Results: Combined treatment with 5-ALA and X-ray irradiation at a dose rate of 2 Gy did not affect the viability of human U87 GBM cells. However, higher dosages of X-ray irradiation (4-8 Gy) in combination with 5-ALA at a concentration of 300 µg/ml significantly decreased the viability of human U87 GBM cells. 5-ALA at both concentrations of 100 and 300 µg/ml significantly and dose-dependently reduced the cell viability of human U87 GBM cells exposed to X-ray irradiation at a dose rate of 14 Gy compared to the control groups. Application of 5-ALA alone significantly reduced the cell viability at both concentrations compared to non-treated controls. Administration of 5-ALA in combination of X-ray irradiation significantly increased the amount of apoptotic cells and SUB-G1 cell-cycle arrest compared to the control groups. Furthermore, co-administration of 5-ALA and X-ray irradiation significantly inhibited the migration ability of human U87 GBM cells.

Conclusion: Our results suggest that treatment with 5-ALA efficiently sensitized human U-87 glioblastoma cells to X-ray irradiation.