gms | German Medical Science

71. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie (DGNC)
9. Joint Meeting mit der Japanischen Gesellschaft für Neurochirurgie

Deutsche Gesellschaft für Neurochirurgie (DGNC) e. V.

21.06. - 24.06.2020

Expression of cyclooxygenase 2 (COX-2) in the wall of human cerebral aneurysms and correlation to MRI

Expression von Cyclooxygenase 2 (COX-2) in der Wand zerebraler Aneurysmen in Korrelation zur MRT-Bildgebung

Meeting Abstract

  • presenting/speaker Jan Rodemerk - Universitätsklinikum Essen, Neurochirurgie, Essen, Deutschland
  • Andreas Junker - Universitätsklinikum Essen, Neuropathologie, Essen, Deutschland
  • Bixia Chen - Universitätsklinikum Essen, Neurochirurgie, Essen, Deutschland; Universität Duisburg-Essen, Erwin L. Hahn Institute for Magnetic Resonance Imaging, Essen, Deutschland
  • Daniela Pierscianek - Universitätsklinikum Essen, Neurochirurgie, Essen, Deutschland
  • Philipp Dammann - Universitätsklinikum Essen, Neurochirurgie, Essen, Deutschland
  • Alexander Radbruch - Universitätsklinikum Essen, Institut für Diagnostische und Interventionelle Radiologie und Neuroradiologie, Essen, Deutschland
  • Harald H. Quick - Universitätsklinikum Essen, Erwin L. Hahn Institute for Magnetic Resonance Imaging, Essen, Deutschland; Universität Duisburg-Essen, Erwin L. Hahn Institute for Magnetic Resonance Imaging, Essen, Deutschland
  • Stefan Maderwald - Universität Duisburg-Essen, Erwin L. Hahn Institute for Magnetic Resonance Imaging, Essen, Deutschland
  • Yuan Zhu - Universitätsklinikum Essen, Molekulare Neurochirurgie, Essen, Deutschland
  • Ramazan Jabbarli - Universitätsklinikum Essen, Neurochirurgie, Essen, Deutschland
  • Ulrich Sure - Universitätsklinikum Essen, Neurochirurgie, Essen, Deutschland
  • Karsten Henning Wrede - Universitätsklinikum Essen, Neurochirurgie, Essen, Deutschland; Universität Duisburg-Essen, Erwin L. Hahn Institute for Magnetic Resonance Imaging, Essen, Deutschland

Deutsche Gesellschaft für Neurochirurgie. 71. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie (DGNC), 9. Joint Meeting mit der Japanischen Gesellschaft für Neurochirurgie. sine loco [digital], 21.-24.06.2020. Düsseldorf: German Medical Science GMS Publishing House; 2020. DocV111

doi: 10.3205/20dgnc113, urn:nbn:de:0183-20dgnc1135

Veröffentlicht: 26. Juni 2020

© 2020 Rodemerk et al.
Dieser Artikel ist ein Open-Access-Artikel und steht unter den Lizenzbedingungen der Creative Commons Attribution 4.0 License (Namensnennung). Lizenz-Angaben siehe http://creativecommons.org/licenses/by/4.0/.


Gliederung

Text

Objective: The pathophysiology of development, growth and rupture of cerebral aneurysms is only partially understood. Cyclooxygenase 2 (COX-2) converts arachidonic acid to PGH2 which in turn is isomerized to PGE2. COX-2 plays an important role in the inflammatory pathway of the human body. Expression of COX-2 seems to be related to aneurysm instability and might serve as a future target for medical treatment and rupture prevention. The aim of this feasibility study was to investigate COX-2 expression in the wall of cerebral aneurysms and correlation to image features in clinical (1-3 Tesla) MRI and ultra-high field 7 Tesla MRI.

Methods: The study group comprised 5 patients with partly thrombosed saccular intracranial Aneurysms (IAs), one ruptured, 4 unruptured which underwent microsurgical treatment. Formaldehyde fixed paraffin embedded samples were immunohistochemically (IHC) stained with a monoclonal antibody against COX-2 (DAKI, Clone: CX-294). Perls’s Prussian blue staining and MRI images were correlated to the IHC, that was analysed with the "Trainable Weka Segmentation" (doi:10.1093/bioinformatics/btx180).

Results: Aneurysm dome size ranged between5 to 40 millimetres. Proportion of COX-2 positive cells ranged between 3.7 % to 79.98 %. The expression of COX-2 correlated positively with aneurysm size, but in the smallest aneurysm which had a relatively high expression of 47.85 % COX-2 positive cells. At all field strength, MRI shows a wall hypointensity due to iron deposition (Figure 2 A, B).

Figure 1 [Fig. 1]

Figure 2 [Fig. 2]

Conclusion: As COX-2 could be a future molecular target for aneurysm treatment the hypointense signal patterns in TOF and SWI have a potential to serve as a biomarker for treatment stratification and treatment response monitoring.