Artikel
Total DNA methylation estimation in tissue and peripheral blood samples as tumour malignancy marker in intracranial meningiomas
Schätzung der Gesamt-DNA-Methylierung in Gewebe- und peripheren Blutproben als Malignitätsmarker bei intrakraniellen Meningeomen
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Autoren
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Anna-Maria Barciszewska
- Karol Marcinkowski University of Medical Sciences, Neurosurgery and Neurotraumatology, Posen, Poland
| Veröffentlicht: | 26. Juni 2020 |
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Gliederung
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Objective: Meningiomas are the most common primary intracranial tumors in adults. They are initially detected with neuroimaging techniques, but definite histological diagnosis requires surgical procedure to collect tumor tissue. Gross total resection is an optimal and final treatment for majority of patients, followed by radiotherapy in malignant or refractory cases. However, there are lots of uncertainties about i.a. need for intervention in incidental cases, estimation of growth kinetics, risk of malignant transformation or response to radiotherapy. It has already been shown that epigenetics plays a crucial role in cancer biology, development and progression. Therefore, we decided to look on meningioma through changes of the most studied epigenetic mark, 5-methylcytosine in DNA.
Methods: We performed the analysis of the total amount of 5-methylcytosine in DNA isolated from intracranial meningioma tissues and peripheral blood samples of the same patients, using thin layer chromatography separation and identification of radioactively labelled nucleotides.
Results: The analyzed cohort consisted of 100 individuals diagnosed with brain meningioma, aged from 27 to 80 years. The most abundant histological variant of meningioma in analyzed cohort was meningothelial, followed by fibrous, angiomatous, transitional, mixed, atypical, anaplastic, and psammomatous types. We found that the 5-methylcytosine level in DNA from intracranial meningiomas changes depending on the malignancy grade. The m5C level in intracranial meningioma DNA negatively correlates with tumor grade. The less malignant tumors show higher m5C contents than more malignant, and the difference is statistically significant. The amount of 5-methylcytosine in tumor tissue and peripheral blood is almost identical. The calculated r correlation coefficient for the whole group of patients with tissue-blood pair was 0.72
Conclusion: The total DNA methylation of intracranial meningiomas can serve as a tool for their characteristics and malignancy estimation. Moreover, while the level of methylation values (R) for tissue and peripheral blood samples from patients with intracranial meningiomas are almost at the same level, that also enables to use peripheral blood as a sample for detecting the tumor or monitoring of the disease.
