gms | German Medical Science

70. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie (DGNC)
Joint Meeting mit der Skandinavischen Gesellschaft für Neurochirurgie

Deutsche Gesellschaft für Neurochirurgie (DGNC) e. V.

12.05. - 15.05.2019, Würzburg

In vitro experiments of ciprofloxacin based 5-ALA photodynamic therapy on human chordoma cell lines

Experimentelle Beobachtungen des Einflusses einer Ciprofloxacin-5-ALA gestützten photodynamischen Therapie an humanen Chordomzelllinien

Meeting Abstract

  • presenting/speaker Hanah Gull - Universitätsklinikum Düsseldorf, Neurochirurgie, Düsseldorf, Deutschland
  • Brigitte Senger - Universitätsklinikum Düsseldorf, Neurochirurgie, Düsseldorf, Deutschland
  • Rüdiger Sorg - Universitätsklinikum Düsseldorf, Institut für Transplantationsdiagnostik und Zelltherapeutika, Düsseldorf, Deutschland
  • Peter Möller - Universitätsklinikum, Pathologie, Ulm, Deutschland
  • Kevin Mellert - Universitätsklinikum, Pathologie, Ulm, Deutschland
  • Hans-Jakob Steiger - Universitätsklinikum Düsseldorf, Neurochirurgie, Düsseldorf, Deutschland
  • Jan Frederick Cornelius - Universitätsklinikum Düsseldorf, Neurochirurgie, Düsseldorf, Deutschland

Deutsche Gesellschaft für Neurochirurgie. 70. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie (DGNC), Joint Meeting mit der Skandinavischen Gesellschaft für Neurochirurgie. Würzburg, 12.-15.05.2019. Düsseldorf: German Medical Science GMS Publishing House; 2019. DocP210

doi: 10.3205/19dgnc545, urn:nbn:de:0183-19dgnc5451

Veröffentlicht: 8. Mai 2019

© 2019 Gull et al.
Dieser Artikel ist ein Open-Access-Artikel und steht unter den Lizenzbedingungen der Creative Commons Attribution 4.0 License (Namensnennung). Lizenz-Angaben siehe http://creativecommons.org/licenses/by/4.0/.


Gliederung

Text

Objective: Chordomas are aggressive tumours of the skull base and sacrum. Complete resection is difficult, and they are known to be chemo- and radioresistant. 5-ALA PDT induces an accumulation of protoporphyrin in haemoglobin metabolism. This increased accumulation of protoporphyrin leads to photodamage and generation of reactive oxygen species in vitro. Furthermore, quinolones, such as ciprofloxacin, function as iron chelators, which downregulate the use of iron to haem biosynthesis intracellularly. In the present study we examined the impact of 5-ALA PDT on different human chordoma cell lines. Furthermore, we investigated the variation of 5-ALA PDT parameters: 5-ALA incubation time and supplemental use of iron chelators such as ciprofloxacin.

Methods: Experiments were accomplished on an in vitro model with two different immortalized human chordoma cell lines; U-CH2 and U-CH2A. After pre-incubation for 24h with varying ciprofloxacin concentrations, cells were incubated with different 5-ALA amounts for additional 4h or 6h and subsequently exposed to PDT. Cells were irradiated by a diode laser for 625 seconds at λ=635 nm with 15,6 J/cm2. For each cell line, two experimental groups were studied: one with ciprofloxacin and 5-ALA, and one with 5-ALA only; negative control groups were also realized. Cell viability was evaluated by WST-1 assay.

Results: Cell viability in both cell lines decreased by using supplemental ciprofloxacin. UCH-2 cell viability was significantly lower at higher ciprofloxacin- 5-ALA concentrations than 5-ALA addition only (ANOVA; p≤0.001 for ≥3ug/ml ciprofloxacin plus ≥30 ug/ml ALA). UCH-2A needed a higher concentration to show significant effects of ciprofloxacin-5-ALA PDT (ANOVA; p≤0.001 for ≥6ug/ml ciprofloxacin plus ≥60 ug/ml ALA). In these examinations, different incubation times of ALA (4h vs. 6h), after pre-incubation for 24h with ciprofloxacin, caused no significant differences in cell viability, with one exception: Cell viability of U-CH2A cell line turned out to be significantly lower, when cells were incubated with 5-ALA for only 4h compared to 6h at higher concentrations (ANOVA; p≤0.01 for ≥8ug/ml ciprofloxacin plus ≥80 ug/ml ALA).

Conclusion: Ciprofloxacin, which is commonly used as antibiotic, improved the effect of 5-ALA PDT on 2 human chordoma cell lines. There was a ciprofloxacin-5-ALA dose-dependent effect, independently from a 4h or 6h 5-ALA incubation time.