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69. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie (DGNC)
Joint Meeting mit der Mexikanischen und Kolumbianischen Gesellschaft für Neurochirurgie

Deutsche Gesellschaft für Neurochirurgie (DGNC) e. V.

03.06. - 06.06.2018, Münster

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Effects of combined neural stem cell therapy and treadmill training on the functional recovery after experimental cervical spinal cord injury

Meeting Abstract

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  • A. Younsi - Heidelberg University Hospital, Department of Neurosurgery, Heidelberg, Deutschland
  • M. Scherer - Heidelberg University Hospital, Department of Neurosurgery, Heidelberg, Deutschland
  • L. Riemann - Heidelberg University Hospital, Department of Neurosurgery, Heidelberg, Deutschland
  • G. Zheng - Heidelberg University Hospital, Department of Neurosurgery, Heidelberg, Deutschland
  • T. Skutella - Heidelberg University Hospital, Department of Neuroanatomy, Heidelberg, Deutschland
  • A. Unterberg - Heidelberg University Hospital, Department of Neurosurgery, Heidelberg, Deutschland
  • K. Zweckberger - Heidelberg University Hospital, Department of Neurosurgery, Heidelberg, Deutschland

Deutsche Gesellschaft für Neurochirurgie. 69. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie (DGNC), Joint Meeting mit der Mexikanischen und Kolumbianischen Gesellschaft für Neurochirurgie. Münster, 03.-06.06.2018. Düsseldorf: German Medical Science GMS Publishing House; 2018. DocBO02

doi: 10.3205/18dgnc243, urn:nbn:de:0183-18dgnc2437

Veröffentlicht: 18. Juni 2018

© 2018 Younsi et al.
Dieser Artikel ist ein Open-Access-Artikel und steht unter den Lizenzbedingungen der Creative Commons Attribution 4.0 License (Namensnennung). Lizenz-Angaben siehe http://creativecommons.org/licenses/by/4.0/.

Gliederung

Text

Background: Neural precursor cell (NPC) transplantation after spinal cord injury (SCI) has shown beneficial effects on neuroregeneration. However, functional recovery may be limited due to astrogliosis and cyst formation. Treadmill training (TT) following SCI is thought to reduce these negative effects. Therefore, combining NPC transplantation and TT after SCI might improve functional recovery.

Methods: 43 Wistar rats received cervical clip-compression SCI at C6 level. Animals were randomized into four treatment groups (NPC + TT, NPC only, vehicle, sham). NPCs were injected into the spinal cord of immunosuppressed rats 10 days after SCI. Daily TT and weekly neurobehavioral tests were performed until the animals were sacrificed for immunohistochemical analysis eight weeks after SCI. Statistical analysis was performed (p < 0.05 was considered significant).

Results: Animals who received NPCs and TT showed significantly higher locomotor function compared to NPC only and vehicle animals 8 weeks after injury in the BBB Score. Gridwalk testing revealed significantly better fine motor skills in the NPC + TT group. Tactile sensitivity wasn’t pathologically altered as examined by the von Frey test. Histologically, NPC + TT treatment lead to significant reduction of astrogliosis and cyst size compared to vehicle animals.

Conclusion: In our study, the combined NPC + TT treatment lead to significant better outcomes in the BBB score and Gridwalk test. Astrogliosis and cyst sizes were significantly reduced when animals were treated with NPC + TT. Collectively, our data suggests that TT may be a beneficial addition to NPC transplantation after SCI.